RCN2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84956-4-RR (same clone as 84956-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84956-4-RR (same clone as 84956-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 84956-4-RR (same clone as 84956-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84956-4-RR (RCN2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84956-4-RR (same clone as 84956-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84956-4-RR (same clone as 84956-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

RCN2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84956-4-PBS

Clone No.242612A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242612A4, Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein

---

Product Information

84956-4-PBS targets RCN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0247

  Product name: Recombinant human RCN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 23-209 aa of BC004892

  Sequence: GKAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE

• Full Name: reticulocalbin 2, EF-hand calcium binding domain
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC004892
• Gene Symbol: RCN2
• Gene ID (NCBI): 5955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14257
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Reticulocalbin-2 (RCN2) is also named as endoplasmic reticulum Ca2+-binding protein of 55 kDa (ERC55) and E6-binding protein (E6BP). It is strictly located in endoplasmic reticulum (PMID: 34094681). RCN2 is a low-affinity Ca2+ binding protein localized to the secretory pathway of mammalian cells, belongs to a member of the EF-hand Ca2+ binding protein family, which participate in the secretory process, chaperone activity, signal transduction as well as participation in a large variety of disease processes (PMID: 19260022). As for cancer research, several studies showed that RCN2 was related to tumor progression, such as mammary cancer, cervical cancer, neck squamous cell carcinoma and colorectal cancer (PMID: 16351756, PMID: 32422974). RCN2 played a pivotal role in HCC pathogenesis through regulating activation of the epidermal growth factor receptor (EGFR) pathway and modulating epidermal growth factor receptor EGFR dimerization and internalization (PMID: 28745317).