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  • KD/KO Validated

REST Polyclonal antibody

REST Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat and More (2)





Cat no : 22242-1-AP


NRSF, REST, X2 box repressor, XBR

Tested Applications

Positive WB detected inRaji cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22242-1-AP targets REST in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REST fusion protein Ag17707
Full Name RE1-silencing transcription factor
Calculated Molecular Weight 1097 aa, 122 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC132859
Gene Symbol REST
Gene ID (NCBI) 5978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).


Product Specific Protocols
WB protocol for REST antibody 22242-1-APDownload protocol
IHC protocol for REST antibody 22242-1-APDownload protocol
IP protocol for REST antibody 22242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

YAP drives fate conversion and chemoresistance of small cell lung cancer.

Authors - Qingzhe Wu
  • KD Validated

Cell Death Differ

The cross talk of two family members of β-TrCP in the regulation of cell autophagy and growth.

Authors - Danrui Cui


REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling.

Authors - Zhiqi Song
  • KD Validated

Cell Death Dis

ALKBH5-mediated m 6 A demethylation of KCNK15-AS1 inhibits pancreatic cancer progression via regulating KCNK15 and PTEN/AKT signaling

Authors - Yuan He

Front Mol Neurosci

Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.

Authors - Zhiqi Song

Mol Neurobiol

Inhibition of the Epigenetic Regulator REST Ameliorates Ischemic Brain Injury.

Authors - Kahlilia C Morris-Blanco
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Aktan (Verified Customer) (12-24-2019)

The product yield satisfactory results with 5%BSA in PBST blocking; primary dilution in the blocking buffer and o/n incubation. The blot is washed and incubated with Licor near IR dye-conjugated goat-antirabbit secondary antibodies for 1h and after washing the bands were detected in Licor.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate cancer cell lines
REST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:22242-1-AP)