REST Polyclonal antibody

• Featured Product
• KD/KO Validated

REST Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 22242-1-AP

Synonyms

NRSF, REST, X2 box repressor, XBR

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Validation Data Gallery

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  WB analysis of Raji using 22242-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22242-1-AP

  WB result of REST antibody (22242-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells.

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  WB analysis of HEK-293 using 22242-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22242-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22242-1-AP

  IP Result of anti-REST (IP:22242-1-AP, 5ug; Detection:22242-1-AP 1:1000) with HeLa cells lysate 2720ug.

• 

  IHC staining of human breast cancer using 22242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Raji cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Product Information

The immunogen of 22242-1-AP is REST Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: REST fusion protein Ag17707
• Full Name: RE1-silencing transcription factor
• Calculated molecular weight: 1097 aa, 122 kDa
• Observed molecular weight: 200 kDa
• GenBank accession number: BC132859
• Gene symbol: REST
• Gene ID (NCBI): 5978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

Publications

Species	Application	Title
mouse		Sci Adv; YAP drives fate conversion and chemoresistance of small cell lung cancer.; Authors - Qingzhe Wu ; KD Validated
human	WB	Cell Death Differ; The cross talk of two family members of β-TrCP in the regulation of cell autophagy and growth.; Authors - Danrui Cui
rat	WB,IF	Oncotarget; REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling.; Authors - Zhiqi Song ; KD Validated
human	WB,CoIP	Cell Death Dis; ALKBH5-mediated m 6 A demethylation of KCNK15-AS1 inhibits pancreatic cancer progression via regulating KCNK15 and PTEN/AKT signaling; Authors - Yuan He
hamster	WB,IF	Front Mol Neurosci; Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.; Authors - Zhiqi Song
mouse	IF	Mol Neurobiol; Inhibition of the Epigenetic Regulator REST Ameliorates Ischemic Brain Injury.; Authors - Kahlilia C Morris-Blanco ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aktan (Verified Customer) (12-24-2019)

The product yield satisfactory results with 5%BSA in PBST blocking; primary dilution in the blocking buffer and o/n incubation. The blot is washed and incubated with Licor near IR dye-conjugated goat-antirabbit secondary antibodies for 1h and after washing the bands were detected in Licor.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Prostate cancer cell lines

REST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:22242-1-AP)