• Featured Product
  • KD/KO Validated

REST Polyclonal antibody

REST Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22242-1-AP

Synonyms

NRSF, REST, X2 box repressor, XBR



Tested Applications

Positive WB detected inRaji cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22242-1-AP is REST Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REST fusion protein Ag17707
Full Name RE1-silencing transcription factor
Calculated molecular weight 1097 aa, 122 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC132859
Gene symbol REST
Gene ID (NCBI) 5978
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

Protocols

Product Specific Protocols
WB protocol for REST antibody 22242-1-APDownload protocol
IHC protocol for REST antibody 22242-1-APDownload protocol
IP protocol for REST antibody 22242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Death Dis

Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury.

Authors - Julie K Bray
human,ratWB

J Mol Cell Cardiol

Remodeling of the m6A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy.

Authors - Scott A Hinger
humanIHC

J Dent Res

Novel REST Truncation Mutations Causing Hereditary Gingival Fibromatosis.

Authors - J T Chen
ratWB,IF

Neuropharmacology

Lithium alleviates neurotoxic prion peptide-induced synaptic damage and neuronal death partially by the upregulation of nuclear target REST and the restoration of Wnt signaling.

Authors - Zhiqi Song
  • KD Validated
mouseIF

Mol Neurobiol

Inhibition of the Epigenetic Regulator REST Ameliorates Ischemic Brain Injury.

Authors - Kahlilia C Morris-Blanco
  • KO Validated
hamsterWB,IF

Front Mol Neurosci

Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.

Authors - Zhiqi Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Aktan (Verified Customer) (12-24-2019)

The product yield satisfactory results with 5%BSA in PBST blocking; primary dilution in the blocking buffer and o/n incubation. The blot is washed and incubated with Licor near IR dye-conjugated goat-antirabbit secondary antibodies for 1h and after washing the bands were detected in Licor.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate cancer cell lines
REST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:22242-1-AP)