REST Polyclonal antibody

• 

  WB analysis of Raji using 22242-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22242-1-AP

  WB result of REST antibody (22242-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells.

• 

  IP experiment of HeLa using 22242-1-AP

  IP result of anti-REST (IP:22242-1-AP, 4ug; Detection:22242-1-AP 1:15000) with HeLa cells lysate 1750 ug.

• 

  IHC staining of human breast cancer using 22242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

REST Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 22242-1-AP

Publications(27)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

XBR, X2 box repressor, NRSF

---

Tested Applications

• Positive WB detected in: Raji cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 8 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Product Information

22242-1-AP targets REST in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: REST fusion protein Ag17707
• Full Name: RE1-silencing transcription factor
• Calculated Molecular Weight: 1097 aa, 122 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC132859
• Gene Symbol: REST
• Gene ID (NCBI): 5978
• RRID: AB_2879044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13127
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

Publications

Species	Application	Title
mouse		Sci Adv; YAP drives fate conversion and chemoresistance of small cell lung cancer.; Authors - Qingzhe Wu ; KD Validated
human	WB	Cell Death Differ; The cross talk of two family members of β-TrCP in the regulation of cell autophagy and growth.; Authors - Danrui Cui
rat	WB,IF	Oncotarget; REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling.; Authors - Zhiqi Song ; KD Validated
human	WB,CoIP	Cell Death Dis; ALKBH5-mediated m 6 A demethylation of KCNK15-AS1 inhibits pancreatic cancer progression via regulating KCNK15 and PTEN/AKT signaling; Authors - Yuan He
hamster	WB,IF	Front Mol Neurosci; Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.; Authors - Zhiqi Song
mouse	IF	Mol Neurobiol; Inhibition of the Epigenetic Regulator REST Ameliorates Ischemic Brain Injury.; Authors - Kahlilia C Morris-Blanco ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Aktan (Verified Customer) (12-24-2019)

The product yield satisfactory results with 5%BSA in PBST blocking; primary dilution in the blocking buffer and o/n incubation. The blot is washed and incubated with Licor near IR dye-conjugated goat-antirabbit secondary antibodies for 1h and after washing the bands were detected in Licor.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Prostate cancer cell lines

REST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:22242-1-AP)