|Positive WB detected in||Raji cells, HEK-293 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22242-1-AP is REST Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||REST fusion protein Ag17707|
|Full Name||RE1-silencing transcription factor|
|Calculated molecular weight||1097 aa, 122 kDa|
|Observed molecular weight||200 kDa|
|GenBank accession number||BC132859|
|Gene ID (NCBI)||5978|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).
Cell Death Dis
Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury.
J Mol Cell Cardiol
Remodeling of the m6A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy.
J Dent Res
Novel REST Truncation Mutations Causing Hereditary Gingival Fibromatosis.
Lithium alleviates neurotoxic prion peptide-induced synaptic damage and neuronal death partially by the upregulation of nuclear target REST and the restoration of Wnt signaling.
Inhibition of the Epigenetic Regulator REST Ameliorates Ischemic Brain Injury.
Front Mol Neurosci
Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Aktan (Verified Customer) (12-24-2019)
The product yield satisfactory results with 5%BSA in PBST blocking; primary dilution in the blocking buffer and o/n incubation. The blot is washed and incubated with Licor near IR dye-conjugated goat-antirabbit secondary antibodies for 1h and after washing the bands were detected in Licor.