ChromoTek RFP Monoclonal antibody (6G6)

Mouse monoclonal antibody [6G6] to Red Fluorescent Proteins

Host/IsoType

Mouse/IgG2c

Specificity

mRFP

Applications

WB

Conjugate

Unconjugated

Clone No.

6G6

Cat no : 6g6

Synonyms

RFP, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

Mouse monoclonal antibody [6G6] to Red Fluorescent Proteins

DescriptionRFP mouse monoclonal [6G6] for WB
ApplicationsWestern blotting
Specificity/TargetTested on mRFP, mCherry, mPlum, mOrange, mRFPruby, DsRed, mScarlet, mScarlet-I, OFP, tdTomato
For the complete list, please click here: Fluorescent protein specificity table antibodies
Recommended DilutionWestern Blot: 1:2,000
Host/IsoTypeMouse/IgG2c
ClassMonoclonal
TypeAntibody
ImmunogenRed fluorescent protein (RFP)
UniProtKBQ9U6Y8
Clone No.6G6
ConjugateUnconjugated
FormLiquid
Purification MethodProtein A or G purified
RRIDAB_2631395
Storage BufferPBS, 50% glycerol, preservative: 0.09% sodium azide.
Storage ConditionShipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment.

Publications

ApplicationTitle
IF

Nature

Trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase.

Authors - Sujata Jha
WB,IF

Nature

POLAR-guided signalling complex assembly and localization drive asymmetric cell division.

Authors - Anaxi Houbaert
IF

Nature

FHL1 is a major host factor for chikungunya virus infection.

Authors - Laurent Meertens
IP

Nature

Structural basis of NPR1 in activating plant immunity.

Authors - Shivesh Kumar

Cell

Deficient Endoplasmic Reticulum-Mitochondrial Phosphatidylserine Transfer Causes Liver Disease.

Authors - María Isabel Hernández-Alvarez
WB

Cell

Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response.

Authors - Raul Zavaliev

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iga (Verified Customer) (08-17-2022)

The picture shows samples of four different mCherry tagged proteins produced by Phytophthora capsici (two independent transgenic lines each time). Each sample was immunoprecipitated from plant tissue infected by transgenic Phytophthora. The correct size of the proteins of interest is indicated by the red arrow. The explanation of the presence of additional bands on western blot: 1) could be that it's the matter of mCherry cleavage that occurs inside Phytophthora mycelium/ 2) modification of protein size that is the consequence of protein maturation inside mycelium/ 3) these are proteins that are secreted by Phytophthora during infection to manipulate plant immunity- it's possible that the host plant, in order to defend from the pathogen, has proteases that cleaves Phytophthora proteins. From my experience I wouldn't interpret these additional bands as the consequence of the revalation with antibody (confocal microscopy pictures showed that the proteins of interest are localised in additional compartments than only extrahaustorial space).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Plant pathogen Phytophthora capsici growing in the host tissue (Nicotiana benthamiana)