ChromoTek RFP Monoclonal antibody (6G6)
Mouse monoclonal antibody [6G6] to Red Fluorescent Proteins
Host/IsoType
Mouse/IgG2c
Specificity
mRFP
Applications
WB
Conjugate
Unconjugated
Clone No.
6G6
147
Cat no : 6g6
Synonyms
Validation Data Gallery
![Dilution series of mCherry in HEK 293T
cell lysate
Primary antibody: [6G6] 1:1,000
Secondary antibody: goat anti-mouse
Alexa Fluor® 647 1:1,000](/products/pictures/Result-6g6.png "Dilution series of mCherry in HEK 293T
cell lysate
Primary antibody: [6G6] 1:1,000
Secondary antibody: goat anti-mouse
Alexa Fluor® 647 1:1,000")
Product Information
Mouse monoclonal antibody [6G6] to Red Fluorescent Proteins
| Description | RFP mouse monoclonal [6G6] for WB |
| Applications | Western blotting |
| Specificity/Target | Tested on mRFP, mCherry, mPlum, mOrange, mRFPruby, DsRed, mScarlet, mScarlet-I, OFP, tdTomato For the complete list, please click here: Fluorescent protein specificity table antibodies |
| Recommended Dilution | Western Blot: 1:2,000 |
| Host/IsoType | Mouse/IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Red fluorescent protein (RFP) |
| UniProtKB | Q9U6Y8 |
| Clone No. | 6G6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A or G purified |
| RRID | AB_2631395 |
| Storage Buffer | PBS, 50% glycerol, preservative: 0.09% sodium azide. |
| Storage Condition | Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment. |
Publications
| Application | Title |
|---|---|
Nature POLAR-guided signalling complex assembly and localization drive asymmetric cell division. | |
Cell Deficient Endoplasmic Reticulum-Mitochondrial Phosphatidylserine Transfer Causes Liver Disease. | |
Cell Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iga (Verified Customer) (08-17-2022) | The picture shows samples of four different mCherry tagged proteins produced by Phytophthora capsici (two independent transgenic lines each time). Each sample was immunoprecipitated from plant tissue infected by transgenic Phytophthora. The correct size of the proteins of interest is indicated by the red arrow. The explanation of the presence of additional bands on western blot: 1) could be that it's the matter of mCherry cleavage that occurs inside Phytophthora mycelium/ 2) modification of protein size that is the consequence of protein maturation inside mycelium/ 3) these are proteins that are secreted by Phytophthora during infection to manipulate plant immunity- it's possible that the host plant, in order to defend from the pathogen, has proteases that cleaves Phytophthora proteins. From my experience I wouldn't interpret these additional bands as the consequence of the revalation with antibody (confocal microscopy pictures showed that the proteins of interest are localised in additional compartments than only extrahaustorial space).
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