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RHOA Polyclonal antibody

RHOA Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 10749-1-AP


ARH12, ARHA, h12, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, MCF-7 cells, PC-12 cells, rat brain tissue, mouse brain tissue, HUVEC cells, NIH/3T3 cells, fetal human brain tissue
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10749-1-AP targets RHOA in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOA fusion protein Ag1141
Full Name ras homolog gene family, member A
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005976
Gene Symbol RHOA
Gene ID (NCBI) 387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the function of RhoA?

Ras homolog gene family, member A (RhoA) is a small GTPase that is involved in cytoskeleton organization.1 It is important in actomyosin contractility, as when RhoA binds to GTP it initiates a cascade of events mediating stress fiber or contractile ring formation, where actin bundles crosslink with other proteins. It is also associated with actin polymerization, making RhoA important in membrane ruffling and cell motility.2 The role of RhoA is essential in cell migration and adhesion.

 What is the cellular localization of RhoA?

In motile cells, a leading edge stretches ahead, stabilizes, the cytoskeleton is reorganized, and the tail of the cell retracts. The role of RhoA in contracting actomyosin has linked RhoA with the tail retraction in this process, but studies into the spatiotemporal kinetics of GTPases have revealed that RhoA is also located at the leading edge of migrating cells.3 This highlights the dual roles of this protein in actin contractility and polymerization.

 What is the role of RhoA in disease?

Though it is not an oncogene, the role of RhoA in adhesion and migration has implicated this protein in cancer biology. Expression of RhoA has been found to be higher in malignant tumors compared to benign tumors or in non-tumor tissue, and more invasive tumors have been found to overexpress RhoA.4 This protein is also implicated in neurodegenerative diseases and aging due to its role in axon guidance, with abnormal localization of RhoA associated with Alzheimer's and Parkinson's pathology.5,6 

1.     Ridley, A. J. & Hall, A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70, 389-399 (1992).

2.     21.   Kurokawa, K. & Matsuda, M. Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling. Mol. Biol. Cell 16, 4294-4303 (2005).

3.     22.   Struckhoff, A. P., Rana, M. K. & Worthylake, R. A. RhoA can lead the way in tumor cell invasion and metastasis. Front. Biosci. (Landmark Ed. 16, 1915-26 (2011).

4.     23.   Mcglasson, S. et al. Rare variants of the 3'-5' DNA exonuclease in early onset TREX1 small vessel stroke [version 1; referees: awaiting peer review]. doi:10.12688/wellcomeopenres.12631.1

5.     24.   Huesa, G. et al. Altered Distribution of RhoA in Alzheimer's Disease and AβPP Overexpressing Mice. J. Alzheimer's Dis. 19, 37-56 (2010).

6.     25.   Hynds, D. L. Subcellular localization of Rho GTPases: implications for axon regeneration. Neural Regen. Res. 10, 1032-3 (2015).


Product Specific Protocols
WB protocol for RHOA antibody 10749-1-APDownload protocol
IF protocol for RHOA antibody 10749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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