• Featured Product
  • KD/KO Validated

RHOA Polyclonal antibody

RHOA Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10749-1-AP

Synonyms

ARH12, ARHA, h12, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA



Tested Applications

Positive WB detected inHL-60 cells, PC-12 cells, HeLa cells, MCF-7 cells, rat brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10749-1-AP targets RHOA in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOA fusion protein Ag1141
Full Name ras homolog gene family, member A
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005976
Gene symbol RHOA
Gene ID (NCBI) 387
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the function of RhoA?

Ras homolog gene family, member A (RhoA) is a small GTPase that is involved in cytoskeleton organization.1 It is important in actomyosin contractility, as when RhoA binds to GTP it initiates a cascade of events mediating stress fiber or contractile ring formation, where actin bundles crosslink with other proteins. It is also associated with actin polymerization, making RhoA important in membrane ruffling and cell motility.2 The role of RhoA is essential in cell migration and adhesion.

 What is the cellular localization of RhoA?

In motile cells, a leading edge stretches ahead, stabilizes, the cytoskeleton is reorganized, and the tail of the cell retracts. The role of RhoA in contracting actomyosin has linked RhoA with the tail retraction in this process, but studies into the spatiotemporal kinetics of GTPases have revealed that RhoA is also located at the leading edge of migrating cells.3 This highlights the dual roles of this protein in actin contractility and polymerization.

 What is the role of RhoA in disease?

Though it is not an oncogene, the role of RhoA in adhesion and migration has implicated this protein in cancer biology. Expression of RhoA has been found to be higher in malignant tumors compared to benign tumors or in non-tumor tissue, and more invasive tumors have been found to overexpress RhoA.4 This protein is also implicated in neurodegenerative diseases and aging due to its role in axon guidance, with abnormal localization of RhoA associated with Alzheimer’s and Parkinson’s pathology.5,6 

1.     Ridley, A. J. & Hall, A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70, 389–399 (1992).

2.     21.   Kurokawa, K. & Matsuda, M. Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling. Mol. Biol. Cell 16, 4294–4303 (2005).

3.     22.   Struckhoff, A. P., Rana, M. K. & Worthylake, R. A. RhoA can lead the way in tumor cell invasion and metastasis. Front. Biosci. (Landmark Ed. 16, 1915–26 (2011).

4.     23.   Mcglasson, S. et al. Rare variants of the 3’-5’ DNA exonuclease in early onset TREX1 small vessel stroke [version 1; referees: awaiting peer review]. doi:10.12688/wellcomeopenres.12631.1

5.     24.   Huesa, G. et al. Altered Distribution of RhoA in Alzheimer’s Disease and AβPP Overexpressing Mice. J. Alzheimer’s Dis. 19, 37–56 (2010).

6.     25.   Hynds, D. L. Subcellular localization of Rho GTPases: implications for axon regeneration. Neural Regen. Res. 10, 1032–3 (2015).

Protocols

Product Specific Protocols
WB protocol for RHOA antibody 10749-1-APDownload protocol
IF protocol for RHOA antibody 10749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer

High Expression of lncRNA AFAP1-AS1 Promotes the Progression of Colon Cancer and Predicts Poor Prognosis.

Authors - Hao Bo
ratWB

J Cell Physiol

Sensory plasticity of carotid body is correlated with oxidative stress in paraventricular nucleus during chronic intermittent hypoxia.

Authors - Pei Liu
humanWB

Cancer Cell Int

A DAAM1 3'-UTR SNP mutation regulates breast cancer metastasis through affecting miR-208a-5p-DAAM1-RhoA axis.

Authors - Jie Mei
humanWB

Cancer Manag Res

Wnt5a induces ROR1 and ROR2 to activate RhoA in esophageal squamous cell carcinoma cells.

Authors - Xuping Wu
mouseWB

Mol Hum Reprod

RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.

Authors - Yu Zhang
humanWB,IF

J Biol Chem

Integrin αvβ3-associated DAAM1 is essential for collagen-induced invadopodia extension and cell haptotaxis in breast cancer cells.

Authors - Ting Yan