ARHGAP32 Polyclonal antibody

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  WB analysis using 15024-1-AP

  HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 15024-1-AP (ARHGAP32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 15024-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15024-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15024-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15024-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARHGAP32 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15024-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ARHGAP32, GC GAP, GRIT, KIAA0712, p200RhoGAP, p250GAP, Rho GTPase activating protein, RICS

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15024-1-AP targets ARHGAP32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGAP32 fusion protein Ag7104
• Full Name: Rho GTPase-activating protein
• Calculated Molecular Weight: 231 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC000277
• Gene Symbol: ARHGAP32
• Gene ID (NCBI): 9743
• RRID: AB_3085448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A7KAX9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP32, also known as RICS, belongs to the PX domain-containing GAP family. ARHGAP32 encodes a neuron-associated GTPase-activating protein that regulates dendritic spine morphology and strength. ARHGAP32 also can interact with RhoA and β-catenin, thereby inhibiting phosphorylation of LIMK/cofilin and promoting epithelial-to-mesenchymal transition (EMT) process. ARHGAP32 is highly expressed in brain and testis (PMID: 30888095, PMID: 32205841).

Publications

Species	Application	Title
mouse	WB	J Nutr Biochem; Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline; Authors - Ping Lu