RMND5A Polyclonal antibody

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  WB analysis using 17559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17559-1-AP (RMND5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RMND5A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17559-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RMD5, Protein RMD5 homolog A, P44CTLH, EC:2.3.2.27, E3 ubiquitin-protein transferase RMND5A

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

17559-1-AP targets RMND5A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RMND5A fusion protein Ag11751
• Full Name: required for meiotic nuclear division 5 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 391 aa, 44 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: BC047668
• Gene Symbol: RMND5A
• Gene ID (NCBI): 64795
• RRID: AB_2878416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H871
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancers (Basel); Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.; Authors - Chunyuan Tang ; KD Validated
human	WB	Chembiochem; Identifying E3 ligase substrates with quantitative degradation proteomics; Authors - Victoria N Jordan
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (09-27-2020)

I used this antibody to screen CRISPR-knock out, and found it difficult to do so (at least in HEK293T cells). There are so so many non-specific bands, and the right MW band was weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

RMND5A Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:17559-1-AP)