Various lysates were subjected to SDS PAGE followed by western blot with 23927-1-AP (RNF146 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 23927-1-AP (RNF146 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary cancer using 23927-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23927-1-AP (RNF146 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23927-1-AP (RNF146 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunohistochemistry (IHC)
IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
23927-1-AP targets RNF146 in WB, IHC, ELISA applications and shows reactivity with human samples.
Various lysates were subjected to SDS PAGE followed by western blot with 23927-1-AP (RNF146 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary cancer using 23927-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23927-1-AP (RNF146 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RNF146 Polyclonal antibody
Catalog Number
23927-1-AP
Citations
-
Dilutions
WB : 1:1000-1:4000 IHC : 1:200-1:800
Applications
WB, IHC, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
