RNF20 Recombinant antibody

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  WB analysis using 85630-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85630-6-RR (RNF20 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-87 MG using 85630-6-RR

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using RNF20 antibody (85630-6-RR, Clone: 243078G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RNF20 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85630-6-RR

Clone No.243078G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

BRE1, BRE1 A, BRE1A, BRE1-A, E3 ubiquitin-protein ligase BRE1A

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells. C2C12 cells
• Positive IF/ICC detected in: U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85630-6-RR targets RNF20 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNF20 fusion protein Ag16066
• Full Name: ring finger protein 20
• Calculated Molecular Weight: 975 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC110585
• Gene Symbol: RNF20
• Gene ID (NCBI): 56254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5VTR2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF20, also named as E3 ubiquitin-protein ligase BRE1A, is a 975 amino acid protein, which contains 1 RING-type zinc finger and belongs to the BRE1 family. RNF20 is a component of the RNF20/40 complex and localizes in the nucleus. RNF20 is a component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B. RNF20 is required for transcriptional activation of Hox genes and recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. RNF20/40 complex monoubiquitinates and stabilizes Eg5. Loss of RNF20/40 results in spindle assembly defects, cell cycle arrest and apoptosis. Spindle assembly role of the RNF20/40 complex, and implicates the RNF20/40-Eg5 axis in breast carcinogenesis, supporting the pursuit of these proteins as potential targets for breast cancer therapeutic interventions.