RNPS1 Polyclonal antibody

• 

  WB analysis using 32844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32844-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RNPS1 Polyclonal Antibody for WB, ELISA

Cat No. 32844-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

E5.1, LDC2, RNA-binding protein with serine-rich domain 1, RNPS 1, SR-related protein LDC2

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells, Ramos cells, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32844-1-AP targets RNPS1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNPS1 fusion protein Ag37888
• Full Name: RNA binding protein S1, serine-rich domain
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC001838
• Gene Symbol: RNPS1
• Gene ID (NCBI): 10921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15287
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The splicing process of mRNA must be highly precise to carry the correct genetic information to the cytoplasm as templates for translation. And this process involves more than 50 proteins in the splicing complex. RNPS1 is a versatile factor that regulates alternative splicing of a number of pre-mRNAs. It participates in both constitutive splicing and in distinctive modulation of alternative splicing in a substrate dependent manner, and involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Its ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro. The molecular weight observed is consistent with what has been described in the literature (PMID: 39687031/PMID: 10449421).