RORB Polyclonal antibody

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  WB analysis using 17635-1-AP

  Mouse retina tissue(positive) and mouse liver tissue (negative) were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse eye using 17635-1-AP

  IP result of anti-RORB (IP:17635-1-AP, 4ug; Detection:17635-1-AP 1:500) with mouse eye tissue lysate 2680 ug.

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  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RORB Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17635-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

NR1F2, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Retinoid-related orphan receptor-beta, RZRB

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Tested Applications

• Positive WB detected in: mouse retina tissue
• Positive IP detected in: mouse eye tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Product Information

17635-1-AP targets RORB in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11910

  Product name: Recombinant human RORB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 106-459 aa of BC051830

  Sequence: EQRQQQSGEAEALARVYSSSISNGLSNLNNETSGTYANGHVIDLPKSEGYYNVDSGQPSPDQSGLDMTGIKQIKQEPIYDLTSVPNLFTYSSFNNGQLAPGITMTEIDRIAQNIIKSHLETCQYTMEELHQLAWQTHTYEEIKAYQSKSREALWQQCAIQITHAIQYVVEFAKRITGFMELCQNDQILLLKSGCLEVVLVRMCRAFNPLNNTVLFEGKYGGMQMFKALGSDDLVNEAFDFAKNLCSLQLTEEEIALFSSAVLISPDRAWLIEPRKVQKLQEKIYFALQHVIQKNHLDDETLAKLIAKIPTITAVCNLHGEKLQVFKQSHPEIVNTLFPPLYKELFNPDCATGCK

• Full Name: RAR-related orphan receptor B
• Calculated Molecular Weight: 459 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC051830
• Gene Symbol: RORB
• Gene ID (NCBI): 6096
• RRID: AB_2180290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology (PMID: 22189870). In addition, NRs that lack identified ligands are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family (PMID: 7935491), which shares a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as the transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone (PMID: 17431400).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Neuron; Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.; Authors - Chao Wang
mouse	IF	PLoS Biol; An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold; Authors - Wenny Wong
mouse	IF	eNeuro; Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.; Authors - Natalia Galindo-Riera
human	WB,ChIP	Cell Adh Migr; Fe3O4 nanoparticles containing gambogic acid inhibit metastasis in colorectal cancer via the RORB/EMILIN1 axis; Authors - Xiaodong Fan
	WB	BMC Genomics; Transcriptomic and proteomic analyses of sclera in lens-induced myopic guinea pigs; Authors - He-Yan Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Etienne (Verified Customer) (12-19-2022)

Did not work at all in human brain organoids (where the target is present)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoids

RORB Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoids (Cat no:17635-1-AP)