RORB Polyclonal antibody

RORB Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17635-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ChIP, ELISA

NR1F2, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Retinoid-related orphan receptor-beta, RZRB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inY79 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17635-1-AP targets RORB in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RORB fusion protein Ag11910 Predict reactive species
Full Name RAR-related orphan receptor B
Calculated Molecular Weight 459 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC051830
Gene Symbol RORB
Gene ID (NCBI) 6096
RRIDAB_2180290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology (PMID: 22189870). In addition, NRs that lack identified ligands are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family (PMID: 7935491), which shares a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as the transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone (PMID: 17431400).

Protocols

Product Specific Protocols
WB protocol for RORB antibody 17635-1-APDownload protocol
IHC protocol for RORB antibody 17635-1-APDownload protocol
IP protocol for RORB antibody 17635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Neuron

Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.

Authors - Chao Wang
mouseIF

PLoS Biol

An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold

Authors - Wenny Wong
mouseIF

eNeuro

Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.

Authors - Natalia Galindo-Riera
humanWB,ChIP

Cell Adh Migr

Fe3O4 nanoparticles containing gambogic acid inhibit metastasis in colorectal cancer via the RORB/EMILIN1 axis

Authors - Xiaodong Fan
WB

BMC Genomics

Transcriptomic and proteomic analyses of sclera in lens-induced myopic guinea pigs

Authors - He-Yan Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Etienne (Verified Customer) (12-19-2022)

Did not work at all in human brain organoids (where the target is present)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/50, 1/200, 1/500
  • Cell Tissue Type: Human Brain Organoids
RORB Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoids (Cat no:17635-1-AP)
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