Tested Applications
| Positive WB detected in | mouse retina tissue |
| Positive IP detected in | mouse eye tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17635-1-AP targets RORB in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11910 Product name: Recombinant human RORB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 106-459 aa of BC051830 Sequence: EQRQQQSGEAEALARVYSSSISNGLSNLNNETSGTYANGHVIDLPKSEGYYNVDSGQPSPDQSGLDMTGIKQIKQEPIYDLTSVPNLFTYSSFNNGQLAPGITMTEIDRIAQNIIKSHLETCQYTMEELHQLAWQTHTYEEIKAYQSKSREALWQQCAIQITHAIQYVVEFAKRITGFMELCQNDQILLLKSGCLEVVLVRMCRAFNPLNNTVLFEGKYGGMQMFKALGSDDLVNEAFDFAKNLCSLQLTEEEIALFSSAVLISPDRAWLIEPRKVQKLQEKIYFALQHVIQKNHLDDETLAKLIAKIPTITAVCNLHGEKLQVFKQSHPEIVNTLFPPLYKELFNPDCATGCK Predict reactive species |
| Full Name | RAR-related orphan receptor B |
| Calculated Molecular Weight | 459 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC051830 |
| Gene Symbol | RORB |
| Gene ID (NCBI) | 6096 |
| RRID | AB_2180290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology (PMID: 22189870). In addition, NRs that lack identified ligands are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family (PMID: 7935491), which shares a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as the transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone (PMID: 17431400).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RORB antibody 17635-1-AP | Download protocol |
| IP protocol for RORB antibody 17635-1-AP | Download protocol |
| WB protocol for RORB antibody 17635-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RORB Polyclonal antibody (Cat# 17635-1-AP) has 8 total citations published in Nature Neuroscience, Neuron, J Sport Health Sci, PLoS Biol, Cell Adh Migr, BMC Genomics, eNeuro, and bioRxiv. Research fields include Alzheimer's disease and tau pathology, cognitive function and cholinergic circuits, cortical development, colorectal cancer metastasis, myopia, and behavioral genetics in mouse models.
| Species | Application | Title |
|---|---|---|
Nat Neurosci Spinal cord Tau pathology induces tactile deficits and cognitive impairment in Alzheimer's disease via dysregulation of CCK neurons | ||
Neuron Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia. | ||
J Sport Health Sci The medial septal-medial habenula cholinergic circuit: A new mechanism of exercise improving cognitive function in AD mice. | ||
PLoS Biol An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold | ||
Cell Adh Migr Fe3O4 nanoparticles containing gambogic acid inhibit metastasis in colorectal cancer via the RORB/EMILIN1 axis | ||
BMC Genomics Transcriptomic and proteomic analyses of sclera in lens-induced myopic guinea pigs |
Reviews
What customers say
One reviewer tested this antibody in human brain organoids by immunofluorescence at dilutions of 1/50, 1/200, and 1/500 (lot 15890) and reported that it did not work at all, despite the target being present in the sample. The review was rated 1 star and posted in December 2022.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Etienne (Verified Customer) (12-19-2022) | Did not work at all in human brain organoids (where the target is present)
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