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  • KD/KO Validated

RPGRIP1L Polyclonal antibody

RPGRIP1L Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 55160-1-AP

Synonyms

CORS3, FTM, JBTS7, KIAA1005, MKS5, Protein fantom, RPGRIP1 like, RPGRIP1 like protein, RPGRIP1L



Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55160-1-AP targets RPGRIP1L in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, canine, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RPGRIP1-like
Calculated molecular weight 151 kDa
GenBank accession numberNM_015272
Gene symbol RPGRIP1L
Gene ID (NCBI) 23322
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

RPGRIP1L, also named as FTM, KIAA1005, belongs to the RPGRIP1 family. It negatively regulates signaling through the G-protein coupled thromboxane A2 receptor. RPGRIP1L may be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis.(PMID:17558409) Defects in RPGRIP1L are the cause of Joubert syndrome type 7 (JBTS7). Defects in RPGRIP1L are the cause of Meckel syndrome type 5 (MKS5). This antibody is specific to RPGRIP1L.

Protocols

Product Specific Protocols
WB protocol for RPGRIP1L antibody 55160-1-APDownload protocol
IHC protocol for RPGRIP1L antibody 55160-1-APDownload protocol
IP protocol for RPGRIP1L antibody 55160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
humanIF

Am J Hum Genet

Mutations in CSPP1, Encoding a Core Centrosomal Protein, Cause a Range of Ciliopathy Phenotypes in Humans.

Authors - Ranad Shaheen

J Cell Biol

TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.

Authors - Elle C Roberson

Dev Cell

Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.

Authors - Quynh P H Nguyen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alice (Verified Customer) (01-25-2019)

Cells fixed in MeOH at -20'C for 5mins.Blocked with 1% w/v Milk/PBSRPGRIP1L antibody diluted in block 1:1000 and co-stained with Acetylated alpha-Tubulin and gamma-tubulin.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: hTERT RPE-1
RPGRIP1L Antibody Immunofluorescence, validation (1:1000 dilution) in hTERT RPE-1 (Cat no:55160-1-AP)