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RPL23 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 16086-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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RPL23 Antibody WB HeLa cells 16086-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 16086-1-AP(RPL23 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours

RPL23 Antibody IHC human brain tissue 16086-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16086-1-AP( RPL23 Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, PC-3 cells
Positive IHC detected in:
human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
RPL23 fusion protein Ag9120
Full name:
ribosomal protein L23
Calculated molecular weight:
140aa,15 kDa
Observed molecular weight:
15 kDa
GenBank accession number:
BC010114
Gene ID (NCBI):
9349
Gene symbol
RPL23
Synonyms
60S ribosomal protein L17, 60S ribosomal protein L23, ribosomal protein L23, rpL17, RPL23

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485].


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