RPL23 Polyclonal antibody

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  WB analysis using 16086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16086-1-AP (RPL23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16086-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16086-1-AP (RPL23 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16086-1-AP

  IP result of anti-RPL23 (IP:16086-1-AP, 4ug; Detection:16086-1-AP 1:3000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16086-1-AP (RPL23 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16086-1-AP (RPL23 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16086-1-AP (RPL23 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16086-1-AP (RPL23 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

RPL23 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16086-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

60S ribosomal protein L17, 60S ribosomal protein L23, ribosomal protein L23, rpL17, RPL23

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Tested Applications

• Positive WB detected in: BxPC-3 cells, PC-3 cells, Jurkat cells, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• RIP: See 1 publications below

Product Information

16086-1-AP targets RPL23 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL23 fusion protein Ag9120
• Full Name: ribosomal protein L23
• Calculated Molecular Weight: 140 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC010114
• Gene Symbol: RPL23
• Gene ID (NCBI): 9349
• RRID: AB_2181432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62829
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485].

Publications

Species	Application	Title
human	WB	Nat Cell Biol; LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency.; Authors - Kaloyan M Tsanov
human,mouse	IHC	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
human	WB	Blood; Defective ribosome assembly in Shwachman-Diamond syndrome.; Authors - Wong Chi C CC
human	WB	Breast Cancer Res; U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression; Authors - Wenrong Zhang
mouse	WB,IHC	Sci Rep; Ribosomal protein L23 negatively regulates cellular apoptosis via the RPL23/Miz-1/c-Myc circuit in higher-risk myelodysplastic syndrome.; Authors - Yuekun Qi ; KD Validated
human	WB,IHC,IF,RIP	Front Oncol; Ribosomal Protein L23 Drives the Metastasis of Hepatocellular Carcinoma via Upregulating MMP9.; Authors - Minli Yang ; KD Validated