RPL23 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPL23 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16086-1-AP

Synonyms

60S ribosomal protein L17, 60S ribosomal protein L23, ribosomal protein L23, rpL17, RPL23

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Validation Data Gallery

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  WB analysis using 16086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16086-1-AP (RPL23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16086-1-AP (RPL23 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, PC-3 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

16086-1-AP targets RPL23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL23 fusion protein Ag9120
• Full Name: ribosomal protein L23
• Calculated molecular weight: 140 aa, 15 kDa
• Observed molecular weight: 15 kDa
• GenBank accession number: BC010114
• Gene symbol: RPL23
• Gene ID (NCBI): 9349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485].

Publications

Species	Application	Title
rat		J Parkinsons Dis Alzheimers Dis; Therapeutic Advantages of Dual Targeting of PPAR-δ and PPAR-γ in an Experimental Model of Sporadic Alzheimer's Disease.; Authors - D Reich
human	IF	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
		Alzheimers Dement (Amst); Altered expression of insulin-degrading enzyme and regulator of calcineurin in the rat intracerebral streptozotocin model and human apolipoprotein E-ε4-associated Alzheimer's disease.; Authors - Büşra Delikkaya
human	WB	BMC Neurosci; Proteomics profiling and pathway analysis of hippocampal aging in rhesus monkeys.; Authors - Shu Meng
human	IHC	Oncol Rep; Triptolide interrupts rRNA synthesis and induces the RPL23‑MDM2‑p53 pathway to repress lung cancer cells.; Authors - Juan Wang
rat	IHC	J Alzheimers Dis; Improved Brain Insulin/IGF Signaling and Reduced Neuroinflammation with T3D-959 in an Experimental Model of Sporadic Alzheimer's Disease.; Authors - Suzanne M de la Monte