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- KD/KO Validated
ribosomal protein L4 Polyclonal antibody
ribosomal protein L4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11302-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat skeletal muscle tissue, K-562 cells, HeLa cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 18 publications below |
| IP | See 1 publications below |
Product Information
11302-1-AP targets ribosomal protein L4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | Arabidopsis, human, mouse, rice |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ribosomal protein L4 fusion protein Ag1828 |
| Full Name | ribosomal protein L4 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC009888 |
| Gene symbol | RPL4 |
| Gene ID (NCBI) | 6124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ribosomal protein L4 antibody 11302-1-AP | Download protocol |
| IHC protocol for ribosomal protein L4 antibody 11302-1-AP | Download protocol |
| IF protocol for ribosomal protein L4 antibody 11302-1-AP | Download protocol |
| IP protocol for ribosomal protein L4 antibody 11302-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation. | ||
Mol Autism Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP. | ||
Cell Rep Ribosome 18S m6A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth. | ||
Biomed Pharmacother LncRNA SNHG7 accelerates the proliferation, migration and invasion of hepatocellular carcinoma cells via regulating miR-122-5p and RPL4. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anita (Verified Customer) (10-30-2020) | 1:1000 dilution works for HeLa cells, not for C. elegans nematodes
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