ribosomal protein L4 Polyclonal antibody

ribosomal protein L4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11302-1-AP

Synonyms

60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4



Tested Applications

Positive WB detected inmouse brain tissue, rat skeletal muscle tissue, K-562 cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11302-1-AP targets ribosomal protein L4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Arabidopsis, human, mouse, rice
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ribosomal protein L4 fusion protein Ag1828
Full Name ribosomal protein L4
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009888
Gene symbol RPL4
Gene ID (NCBI) 6124
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Protocols

Product Specific Protocols
WB protocol for ribosomal protein L4 antibody 11302-1-APDownload protocol
IHC protocol for ribosomal protein L4 antibody 11302-1-APDownload protocol
IF protocol for ribosomal protein L4 antibody 11302-1-APDownload protocol
IP protocol for ribosomal protein L4 antibody 11302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

RNA helicase A is not required for RISC activity.

Authors - Liang Xue-Hai XH
humanWB

Translation (Austin)

Characterizing inactive ribosomes in translational profiling.

Authors - Botao Liu
human,mouseWB

Cell Rep

Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.

Authors - Kim Schneider
mouseWB

Mol Autism

Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.

Authors - Annie Hien
humanWB

Biomed Pharmacother

LncRNA SNHG7 accelerates the proliferation, migration and invasion of hepatocellular carcinoma cells via regulating miR-122-5p and RPL4.

Authors - Xue Yang
humanWB

Mol Biol Cell

Kinesin molecular motor Eg5 functions during polypeptide synthesis.

Authors - Bartoli Kristen M KM

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anita (Verified Customer) (10-30-2020)

1:1000 dilution works for HeLa cells, not for C. elegans nematodes

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
ribosomal protein L4 Antibody Western Blot validation (1:1000 dilution) in (Cat no:11302-1-AP)