|Positive WB detected in||mouse brain tissue, rat skeletal muscle tissue, K-562 cells, HeLa cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11302-1-AP targets ribosomal protein L4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Arabidopsis, human, mouse, rice|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ribosomal protein L4 fusion protein Ag1828|
|Full Name||ribosomal protein L4|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC009888|
|Gene ID (NCBI)||6124|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.
|Product Specific Protocols|
|WB protocol for ribosomal protein L4 antibody 11302-1-AP||Download protocol|
|IHC protocol for ribosomal protein L4 antibody 11302-1-AP||Download protocol|
|IF protocol for ribosomal protein L4 antibody 11302-1-AP||Download protocol|
|IP protocol for ribosomal protein L4 antibody 11302-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta
RNA helicase A is not required for RISC activity.
Characterizing inactive ribosomes in translational profiling.
Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.
Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.
LncRNA SNHG7 accelerates the proliferation, migration and invasion of hepatocellular carcinoma cells via regulating miR-122-5p and RPL4.
Mol Biol Cell
Kinesin molecular motor Eg5 functions during polypeptide synthesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anita (Verified Customer) (10-30-2020)
1:1000 dilution works for HeLa cells, not for C. elegans nematodes