Ribosomal protein L4 Polyclonal antibody

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  WB analysis using 11302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11302-1-AP

  WB result of Ribosomal protein L4 antibody (11302-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HeLa cells.

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  WB analysis of rat skeletal muscle using 11302-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11302-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11302-1-AP

  IP result of anti-Ribosomal protein L4 (IP:11302-1-AP, 4ug; Detection:11302-1-AP 1:6000) with HeLa cells lysate 1050 ug.

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  IHC staining of human lymphoma using 11302-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11302-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ribosomal protein L4 antibody (11302-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HeLa using 11302-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Ribosomal protein L4 (11302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Ribosomal protein L4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11302-1-AP

Publications(37)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

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Tested Applications

• Positive WB detected in: HeLa cells, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 35 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• RIP: See 1 publications below

Product Information

11302-1-AP targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rice, arabidopsis
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ribosomal protein L4 fusion protein Ag1828
• Full Name: ribosomal protein L4
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC009888
• Gene Symbol: Ribosomal protein L4
• Gene ID (NCBI): 6124
• RRID: AB_2181909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36578
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Publications

Species	Application	Title
	WB	Science; The Ccr4-Not complex monitors the translating ribosome for codon optimality.; Authors - Robert Buschauer
human	WB	Cell; Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate.; Authors - Colin Chih-Chien Wu
mouse	IF	Cell Res; Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.; Authors - Xiaochen Gai
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	WB	Mol Cell; N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.; Authors - Jun Zhou
human	WB	Mol Cell; The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.; Authors - Cindy Meyer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anita (Verified Customer) (10-30-2020)

1:1000 dilution works for HeLa cells, not for C. elegans nematodes

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

Ribosomal protein L4 Antibody Western Blot validation (1:1000 dilution) in (Cat no:11302-1-AP)