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ribosomal protein L4 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 11302-1-AP

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Con: 27 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ribosomal protein L4 Antibody WB mouse brain tissue 11302-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP (ribosomal protein L4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ribosomal protein L4 Antibody IP K-562 cells 11302-1-AP

IP Result of anti-ribosomal protein L4 (IP:11302-1-AP, 3ug; Detection:11302-1-AP 1:800) with K-562 cells lysate 6000ug.

ribosomal protein L4 Antibody IHC human lymphoma tissue 11302-1-AP

Immunohistochemical of paraffin-embedded human lymphoma using 11302-1-AP(ribosomal protein L4 antibody) at dilution of 1:100 (under 10x lens)

ribosomal protein L4 Antibody IF HepG2 cells 11302-1-AP

Immunofluorescent analysis of HepG2 cells, using RPL4 antibody 11302-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Species specificity:
human, mouse, rat, Arabidopsis, rice
Positive WB detected in:
mouse brain tissue, rat skeletal muscle tissue, K-562 cells, HeLa cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ribosomal protein L4 fusion protein Ag1828
Full name:
ribosomal protein L4
Calculated molecular weight:
48 kDa
Observed molecular weight:
48 kDa
GenBank accession number:
BC009888
Gene ID (NCBI):
6124
Gene symbol
RPL4
Synonyms
60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4

Background

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.


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