RPS19 Polyclonal antibody

RPS19 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 15085-1-AP

Synonyms

40S ribosomal protein S19, DBA, ribosomal protein S19, RPS19

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Validation Data Gallery

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  WB analysis using 15085-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15085-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 15085-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15085-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15085-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15085-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15085-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15085-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15085-1-AP (RPS19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15085-1-AP

  IP result of anti-RPS19 (IP:15085-1-AP, 4ug; Detection:15085-1-AP 1:500) with HEK-293 cells lysate 880ug.

• 

  IHC staining of human colon cancer using 15085-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15085-1-AP (RPS19 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 15085-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15085-1-AP (RPS19 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Caco-2 cells, human testis tissue, HEK-293 cells, rat colon tissue, mouse testis tissue, HeLa cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15085-1-AP targets RPS19 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS19 fusion protein Ag7164
• Full Name: ribosomal protein S19
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC000023
• Gene Symbol: RPS19
• Gene ID (NCBI): 6223
• RRID: AB_2180202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes. The RPS19 protein is a component of the 40S ribosomal subunit [PMID: 17726054]. Diamond-Blackfan anemia (DBA) is a congenital erythroid hypoplasia caused by a functional haploinsufficiency of genes encoding for ribosomal proteins. Among these genes, ribosomal protein S19 (RPS19) is mutated most frequently [PMID:21989989]. It also a p53-related ribosomal protein that possibly involves cellular apoptosis through the BAX/p53 pathway [PMID:22272377].

Publications

Species	Application	Title
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy
	WB,IF	Development; Msl3 promotes germline stem cell differentiation in female Drosophila.; Authors - Alicia McCarthy
mouse	IHC	J Proteome Res; Human SOD1-G93A specific distribution evidenced in murine brain of a transgenic model for Amyotrophic Lateral Sclerosis by MALDI Imaging Mass Spectrometry.; Authors - Elena Acquadro
human	WB	Sci Rep; RNA G-quadruplex structures control ribosomal protein production; Authors - DhavalVarshney
human	WB	BBA Clin; Mutation of EMG1 causing Bowen-Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay.; Authors - Joy Armistead
mouse	WB	Adv Sci (Weinh); Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.; Authors - Zhiyuan Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (04-06-2020)

RPS19 is an abundant protein. I could easily detect a clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

RPS19 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:15085-1-AP)