Tested Applications
| Positive WB detected in | Caco-2 cells, human testis tissue, HEK-293 cells, rat colon tissue, mouse testis tissue, HeLa cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15085-1-AP targets RPS19 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7164 Product name: Recombinant human RPS19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC000023 Sequence: MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYGGRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH Predict reactive species |
| Full Name | ribosomal protein S19 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC000023 |
| Gene Symbol | RPS19 |
| Gene ID (NCBI) | 6223 |
| RRID | AB_2180202 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P39019 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes. The RPS19 protein is a component of the 40S ribosomal subunit [PMID: 17726054]. Diamond-Blackfan anemia (DBA) is a congenital erythroid hypoplasia caused by a functional haploinsufficiency of genes encoding for ribosomal proteins. Among these genes, ribosomal protein S19 (RPS19) is mutated most frequently [PMID:21989989]. It also a p53-related ribosomal protein that possibly involves cellular apoptosis through the BAX/p53 pathway [PMID:22272377].
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPS19 antibody 15085-1-AP | Download protocol |
| IP protocol for RPS19 antibody 15085-1-AP | Download protocol |
| WB protocol for RPS19 antibody 15085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS19 Polyclonal Antibody (Cat# 15085-1-AP) has 15 total citations. These appear in journals including Nature Communications, Molecular Cell, Advanced Science, Cell Reports, EBioMedicine, Journal of Translational Medicine, Neural Regeneration Research, Scientific Reports, Development, Journal of Proteome Research, bioRxiv, and BBA Clinical. Associated research fields span ribosome biogenesis, erythroid differentiation, integrated stress response, viral translation, leukemia, pancreatic cancer metastasis, neurodegeneration, G-quadruplex biology, and germline stem cell development.
| Species | Application | Title |
|---|---|---|
Nat Commun DDX41 resolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death | ||
Nat Commun Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs | ||
Adv Sci (Weinh) Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation. | ||
EBioMedicine Skeletal muscle reprogramming of metabolic, ribosomal and developmental pathways contributes to Roux-en-Y gastric bypass-induced adaptation in women. | ||
J Transl Med Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice. |
Reviews
What customers say
The single review for 15085-1-AP is a 5-star rating from April 2020. The user performed Western Blot on HEK293T cells at a 1:1000 dilution and reported that RPS19, being an abundant protein, produced a clear and easily detectable band. Overall, the reviewer was satisfied with the antibody's performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (04-06-2020) | RPS19 is an abundant protein. I could easily detect a clear band.
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