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Raptor Polyclonal antibody

Raptor Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 20984-1-AP


KIAA1303, raptor, RPTOR

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, mouse liver tissue
Positive IP detected inHEK-293 cells
Positive IF detected inU2OS cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20984-1-AP targets Raptor in WB, IP, IF, PLA, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name raptor
Calculated Molecular Weight 149 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberNM_020761
Gene Symbol Raptor
Gene ID (NCBI) 57521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.


Product Specific Protocols
WB protocol for Raptor antibody 20984-1-APDownload protocol
IF protocol for Raptor antibody 20984-1-APDownload protocol
IP protocol for Raptor antibody 20984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto

Mol Cell

Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.

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Nat Commun

Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.

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Nat Commun

N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.

Authors - Hui Han

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Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts

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