CD45 Polyclonal antibody

CD45 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20103-1-AP

Synonyms

B220, CD45, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Tested Applications

Positive WB detected inmouse thymus tissue, rat thymus tissue, mouse spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman colon tissue, human tonsil tissue, human lymphoma tissue, human tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20103-1-AP is CD45 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity Bovine, dog, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD45 fusion protein Ag13712
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
Observed molecular weight 180-220 kDa
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596).  

What is the molecular weight of CD45?

The molecular weight of CD45 is a 180-240 kDa. 

What are the isoforms of CD45?

Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720). 

What is the tissue specificity of CD45?

CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253). 

What are the post-translational modifications of CD45?

CD45 can be heavily N- and O-glycosylated (PMID: 1702721). 

What is CD45’s involvement in disease?

Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).


Protocols

Product Specific Protocols
WB protocol for CD45 antibody 20103-1-APDownload protocol
IHC protocol for CD45 antibody 20103-1-APDownload protocol
IP protocol for CD45 antibody 20103-1-APDownload protocol
FC protocol for CD45 antibody 20103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Metab

PMID: 30837196

Authors - Haixia Xu
ratIHC

Am J Physiol Heart Circ Physiol

NRP1 regulates HMGB1 in vascular endothelial cells under high homocysteine condition.

Authors - Yeshuo Ma
BovineIF

J Exp Zool B Mol Dev Evol

Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.

Authors - Xin-Xin Zhao
mouseIHC

Cells

Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice.

Authors - Felix Hempel
mouseIHC

Cardiovasc Diagn Ther

Kallistatin correlates with inflammation in abdominal aortic aneurysm and suppresses its formation in mice.

Authors - Yuchen He
humanIHC

Ann Transl Med

Increased m6A methylation level is associated with the progression of human abdominal aortic aneurysm.

Authors - Yuchen He