|Positive WB detected in||mouse thymus tissue, HepG2 cells, MCF-7 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22503-1-AP is CNOT9 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CNOT9 fusion protein Ag18197|
|Full Name||RCD1 required for cell differentiation1 homolog (S. pombe)|
|Calculated molecular weight||299 aa, 34 kDa|
|Observed molecular weight||30-40 kDa|
|GenBank accession number||BC007102|
|Gene ID (NCBI)||9125|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.
|Product Specific Protocols|
|WB protocol for CNOT9 antibody 22503-1-AP||Download protocol|
|IHC protocol for CNOT9 antibody 22503-1-AP||Download protocol|
|IF protocol for CNOT9 antibody 22503-1-AP||Download protocol|
|IP protocol for CNOT9 antibody 22503-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex.
Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
Translational control of ERK signaling through miRNA/4EHP-directed silencing.
Nucleic Acids Res
Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution.
Structural and Biochemical Insights to the Role of the CCR4-NOT Complex and DDX6 ATPase in MicroRNA Repression.
A DDX6-CNOT1 Complex and W-Binding Pockets in CNOT9 Reveal Direct Links between miRNA Target Recognition and Silencing.