Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, mouse thymus tissue, HepG2 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human stomach cancer tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22503-1-AP targets CNOT9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18197 Product name: Recombinant human RQCD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-269 aa of BC007102 Sequence: MHSLATAAPVPTTLAQVDREKIYQWINELSSPETRENALLELSKKRESVPDLAPMLWHSFGTIAALLQEIVNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSLGVTGALVKTDEQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVAMILGKMVLQLSKEPSARLLKHVVRCYLRLSDN Predict reactive species |
| Full Name | RCD1 required for cell differentiation1 homolog (S. pombe) |
| Calculated Molecular Weight | 299 aa, 34 kDa |
| Observed Molecular Weight | 30-40 kDa |
| GenBank Accession Number | BC007102 |
| Gene Symbol | CNOT9 |
| Gene ID (NCBI) | 9125 |
| RRID | AB_11232413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92600 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CNOT9 antibody 22503-1-AP | Download protocol |
| IHC protocol for CNOT9 antibody 22503-1-AP | Download protocol |
| IP protocol for CNOT9 antibody 22503-1-AP | Download protocol |
| WB protocol for CNOT9 antibody 22503-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CNOT9 (RQCD1) polyclonal antibody (Cat# 22503-1-AP) has 23 citations across 15 journals, including Nature, Cell, Nature Communications, Molecular Cell, Nucleic Acids Research, Science Advances, and Nature Structural & Molecular Biology. Research fields span miRNA-mediated gene silencing, CCR4-NOT complex function, mRNA decay, antiviral immunity, epigenetic regulation, genome stability, and liver biology. Cited applications include Western blotting, immunoprecipitation, and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Nat Commun RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.
| ||
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. | ||
Nat Commun MARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA |
Reviews
What customers say
Both reviewers gave this antibody a perfect 5-star rating. One user confirmed reliable Western blot performance at 1/1000 dilution (overnight at 4°C) in HeLa cells for CNOT9 detection, further validated by siRNA-mediated knockdown. Another reported successful immunofluorescence at 1:100 dilution (overnight at 4°C) in RPE-1 cells. Overall, users find this antibody effective across applications with straightforward overnight incubation protocols.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-13-2024) | Works well at 1/1000 at 4°C overnight to detect CNOT9 by WB. (also validated through siRNA-mediated KD of CNOT9)
|
Abe (Verified Customer) (04-23-2024) | Worked well at 1:100 overnight at 4c.
|
