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CNOT9 Polyclonal antibody
CNOT9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 22503-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse thymus tissue, HepG2 cells, MCF-7 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
The immunogen of 22503-1-AP is CNOT9 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CNOT9 fusion protein Ag18197 |
Full Name | RCD1 required for cell differentiation1 homolog (S. pombe) |
Calculated molecular weight | 299 aa, 34 kDa |
Observed molecular weight | 30-40 kDa |
GenBank accession number | BC007102 |
Gene symbol | RQCD1 |
Gene ID (NCBI) | 9125 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.
Protocols
Product Specific Protocols | |
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WB protocol for CNOT9 antibody 22503-1-AP | Download protocol |
IHC protocol for CNOT9 antibody 22503-1-AP | Download protocol |
IF protocol for CNOT9 antibody 22503-1-AP | Download protocol |
IP protocol for CNOT9 antibody 22503-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Genes (Basel) Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex. | ||
iScience The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex. | ||
Cell Rep Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription. | ||
Nucleic Acids Res Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution. | ||
Nat Commun RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.
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