Tested Applications
| Positive WB detected in | fetal human brain tissue, A549 cells, mouse spinal cord tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse stomach tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28089-1-AP targets RSAD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig, canine, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27733 Product name: Recombinant human RSAD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 181-361 aa of BC017969 Sequence: DSFDEEVNVLIGRGQGKKNHVENLQKLRRWCRDYRVAFKINSVINRFNVEEDMTEQIKALNPVRWKVFQCLLIEGENCGEDALREAERFVIGDEEFERFLERHKEVSCLVPESNQKMKDSYLILDEYMRFLNCRKGRKDPSKSILDVGVEEAIKFSGFDEKMFLKRGGKYIWSKADLKLDW Predict reactive species |
| Full Name | radical S-adenosyl methionine domain containing 2 |
| Calculated Molecular Weight | 361 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC017969 |
| Gene Symbol | RSAD2 |
| Gene ID (NCBI) | 91543 |
| RRID | AB_2881057 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXG1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RSAD2 antibody 28089-1-AP | Download protocol |
| IP protocol for RSAD2 antibody 28089-1-AP | Download protocol |
| WB protocol for RSAD2 antibody 28089-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RSAD2 Polyclonal antibody (Cat# 28089-1-AP) has 30 citations across 25 journals including Cell, Nature Communications, Nature Microbiology, Molecular Cell, EMBO Reports, and FASEB Journal. Research fields span antiviral immunity and interferon signaling, viral replication (HCV, influenza, SARS-CoV-2, Ebola), autophagy, cancer immunology, lipid metabolism, and innate immune responses.
| Species | Application | Title |
|---|---|---|
Cell Rift Valley fever virus coordinates the assembly of a programmable E3 ligase to promote viral replication | ||
Drug Resist Updat Functional precision oncology platform of BRAF(V600E)-mutated colorectal cancer organoids predicts therapy response and reveals RNF43-mediated immunogenicity. | ||
Nat Commun The potential of H5N1 viruses to adapt to bovine cells varies throughout evolution. | ||
Nat Commun ATG14 targets lipid droplets and acts as an autophagic receptor for syntaxin18-regulated lipid droplet turnover | ||
Mol Cell Histone methylation antagonism drives tumor immune evasion in squamous cell carcinomas |
