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  • KD/KO Validated

RUVBL1 Polyclonal antibody

RUVBL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 10210-2-AP


49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A

Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10210-2-AP targets RUVBL1 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUVBL1 fusion protein Ag0281
Full Name RuvB-like 1 (E. coli)
Calculated Molecular Weight 456 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002993
Gene Symbol RUVBL1
Gene ID (NCBI) 8607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.


Product Specific Protocols
WB protocol for RUVBL1 antibody 10210-2-APDownload protocol
IHC protocol for RUVBL1 antibody 10210-2-APDownload protocol
IF protocol for RUVBL1 antibody 10210-2-APDownload protocol
IP protocol for RUVBL1 antibody 10210-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Immunol

Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families.

Authors - Yi Zhong

Mol Cell

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Authors - Sang Gyun Kim

Nat Struct Mol Biol

R loops regulate promoter-proximal chromatin architecture and cellular differentiation.

Authors - Poshen B Chen

Sci Adv

Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.

Authors - Fabian Zimmermann
  • KD Validated

Sci Adv

Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Authors - Seung Ho Shin
  • KD Validated

Genes Dev

SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.

Authors - Ailone Tichon


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Deyong (Verified Customer) (03-11-2022)

It worked well in H1 human embryonic stem cell.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human embryonic stem cell