|Positive WB detected in||HEK-293 cells, HeLa cells, rat testis tissue, HT-1080 cells, mouse heart tissue, K-562 cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse liver tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10210-2-AP targets RUVBL1 in WB, IP, IHC, IF, CHIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RUVBL1 fusion protein Ag0281|
|Full Name||RuvB-like 1 (E. coli)|
|Calculated molecular weight||456 aa, 50 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC002993|
|Gene ID (NCBI)||8607|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.
Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.
SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.
Biochem Biophys Res Commun
RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis.
Biochim Biophys Acta
Revealing A-Raf functions through its interactome.
Mol Cell Pediatr
Targeted deletion of Ruvbl1 results in severe defects of epidermal development and perinatal mortality.
Cell Biochem Funct
Reptin regulates insulin-stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP-1/PTPN6.