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- KD/KO Validated
RXRA Polyclonal antibody
RXRA Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 21218-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF7 cells, MCF-7 cells, rat liver tissue |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 15 publications below |
IHC | See 2 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
ChIP | See 2 publications below |
RIP | See 1 publications below |
Product Information
The immunogen of 21218-1-AP is RXRA Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RXRA fusion protein Ag15651 |
Full Name | retinoid X receptor, alpha |
Calculated molecular weight | 462 aa, 51 kDa |
Observed molecular weight | 50-54 kDa |
GenBank accession number | BC110998 |
Gene symbol | RXRA |
Gene ID (NCBI) | 6256 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed oftandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in liver, also expressed in lung, kidney and heart. It specifically recognizes the 51kd human RXRA protein.
Protocols
Product Specific Protocols | |
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WB protocol for RXRA antibody 21218-1-AP | Download protocol |
IHC protocol for RXRA antibody 21218-1-AP | Download protocol |
IF protocol for RXRA antibody 21218-1-AP | Download protocol |
IP protocol for RXRA antibody 21218-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Drug Metab Dispos Activation of PXR-Cytochrome P450s axis: A Possible Reason for the Enhanced Accelerated Blood Clearance Phenomenon of PEGylated Liposomes in vivo. | ||
Hum Reprod Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis. | ||
Sci Adv Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment. | ||
Biochem Biophys Res Commun Activation of RAS/ERK alone is insufficient to inhibit RXRα function and deplete retinoic acid in hepatocytes. | ||
Biomed Pharmacother Nrf2 activation is required for curcumin to induce lipocyte phenotype in hepatic stellate cells. | ||
Chin Med Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis. |