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RXRA Polyclonal antibody
RXRA Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 21218-1-AP
Validation Data Gallery
|Positive WB detected in||MCF7 cells, MCF-7 cells, rat liver tissue|
|Positive IP detected in||MCF-7 cells, HCT 116 cells|
|Positive IHC detected in||human kidney tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
21218-1-AP targets RXRA in WB, RIP, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig, duck|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RXRA fusion protein Ag15651|
|Full Name||retinoid X receptor, alpha|
|Calculated molecular weight||462 aa, 51 kDa|
|Observed molecular weight||50-54 kDa|
|GenBank accession number||BC110998|
|Gene ID (NCBI)||6256|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver, and also expressed in the lungs, kidneys, and heart. It can recognize the the mature 54 kDa RXRA and the truncated 44 kD RXRA (PMID: 20541701).
|Product Specific Protocols|
|WB protocol for RXRA antibody 21218-1-AP||Download protocol|
|IHC protocol for RXRA antibody 21218-1-AP||Download protocol|
|IF protocol for RXRA antibody 21218-1-AP||Download protocol|
|IP protocol for RXRA antibody 21218-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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