RXRA Polyclonal antibody

• Featured Product
• KD/KO Validated

RXRA Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 21218-1-AP

Synonyms

NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA

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Validation Data Gallery

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  WB analysis of MCF-7 using 21218-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 21218-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21218-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 21218-1-AP

  IP Result of anti-RXRA (IP:21218-1-AP, 4ug; Detection:21218-1-AP 1:600) with MCF-7 cells lysate 800ug.

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  IHC staining of human kidney using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21218-1-AP

  Immunofluorescent analysis of Hela cells, using RXRA antibody 21218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: MCF7 cells, MCF-7 cells, rat liver tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below
• RIP: See 1 publications below

Product Information

The immunogen of 21218-1-AP is RXRA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RXRA fusion protein Ag15651
• Full Name: retinoid X receptor, alpha
• Calculated molecular weight: 462 aa, 51 kDa
• Observed molecular weight: 50-54 kDa
• GenBank accession number: BC110998
• Gene symbol: RXRA
• Gene ID (NCBI): 6256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed oftandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in liver, also expressed in lung, kidney and heart. It specifically recognizes the 51kd human RXRA protein.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Nat Genet; Comprehensive characterization of protein-protein interactions perturbed by disease mutations.; Authors - Feixiong Cheng
human	ChIP	Lab Anim (NY); Animal models of male subfertility targeted on LanCL1-regulated spermatogenic redox homeostasis.; Authors - Chao Huang
human	WB	Cancer Res; Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.; Authors - Ming Cui
mouse	RIP	Theranostics; Oscillating lncRNA Platr4 regulates NLRP3 inflammasome to ameliorate nonalcoholic steatohepatitis in mice.; Authors - Yanke Lin ; KD Validated
	WB	Antioxid Redox Signal; Manganese (Mn)-induced oxidative stress contributes to intestinal lipid deposition via the deacetylation of PPARγ at K339 by SIRT1.; Authors - Wu-Hong Lv