Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human heart tissue, human brain tissue, human placenta tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19765-1-AP targets RYR2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ryanodine receptor 2 (cardiac) |
| Calculated Molecular Weight | 565 kDa |
| Observed Molecular Weight | 565 kDa |
| GenBank Accession Number | NM_001035 |
| Gene Symbol | RYR2 |
| Gene ID (NCBI) | 6262 |
| RRID | AB_10637859 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP). This antibody detects a band at ~250 kDa which has been reported (PMID: 22962011).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RYR2 antibody 19765-1-AP | Download protocol |
| IHC protocol for RYR2 antibody 19765-1-AP | Download protocol |
| WB protocol for RYR2 antibody 19765-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RYR2 Antibody (Cat# 19765-1-AP) has 38 total citations appearing in journals such as PNAS, Cell Death & Differentiation, EMBO Journal, FASEB Journal, Molecular Metabolism, J Mol Cell Cardiol, Cardiovasc Diabetol, Sci China Life Sci, Free Radic Biol Med, and others. Research fields include cardiovascular disease (arrhythmia, myocardial infarction, heart failure), calcium homeostasis/signaling, neurology, oncology, environmental toxicology, and metabolic regulation.
| Species | Application | Title |
|---|---|---|
MedComm (2020) Spatial Transcriptional Heterogeneity in the Infarct Core and Its Surrounding Regions Targeting Piezo1 Signals in Rats With Myocardial Ischemia-Reperfusion Injury. | ||
Cell Death Differ NAT10 regulates heart development and function by maintaining the expression of genes related to fatty acid β-oxidation and heart contraction | ||
Phytomedicine Divaroside alleviates barium chloride-induced arrhythmia by activating the Nrf2/HO-1 axis to modulate autophagy and calcium homeostasis. | ||
Part Fibre Toxicol Oxygenated polycyclic aromatic hydrocarbons from ambient particulate matter induce electrophysiological instability in cardiomyocytes. | ||
Sci China Life Sci LncRNA CCRR maintains Ca2+ homeostasis against myocardial infarction through the FTO-SERCA2a pathway |
Reviews
What customers say
The product has received one review with a 5-star rating. The reviewer used it for Western Blot on rat heart tissue (lot 00119668) and reported that it works well for this application. Overall, the feedback is positive, confirming reliable performance in WB on rat samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (02-09-2026) | Works well for western blot on rat heart
 |
