RYR2 Polyclonal antibody

RYR2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 19765-1-AP

Synonyms

ARVC2, ARVD2, ryanodine receptor 2 (cardiac), ryanodine receptor 2, cardiac, RYR2, VTSIP



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human placenta tissue, mouse brain tissue
Positive IHC detected inhuman heart tissue, human brain tissue, human placenta tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19765-1-AP targets RYR2 in WB, IHC, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ryanodine receptor 2 (cardiac)
Calculated molecular weight 565 kDa
Observed molecular weight 250 kDa
GenBank accession numberNM_001035
Gene symbol RYR2
Gene ID (NCBI) 6262
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP). This antibody detects a band at ~250 kDa which has been reported (PMID: 22962011).

Protocols

Product Specific Protocols
WB protocol for RYR2 antibody 19765-1-APDownload protocol
IHC protocol for RYR2 antibody 19765-1-APDownload protocol
IF protocol for RYR2 antibody 19765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Sci Rep

The harmful effects of acute PM2.5 exposure to the heart and a novel preventive and therapeutic function of CEOs.

Authors - Lu Dong
ratWB

Cell Biochem Funct

Astragaloside IV attenuates chronic intermittent hypoxia-induced myocardial injury by modulating Ca2+ homeostasis.

Authors - Shan Jiang
ratWB

J Ethnopharmacol

Applyingcooperative module pair analysis to uncover compatibility mechanism of Fangjis: an example of Wenxin Keli decoction.

Authors - Pengqian Wang
mouseWB

Front Cardiovasc Med

Fibroblast Growth Factor 21 Protects Against Atrial Remodeling via Reducing Oxidative Stress

Authors - Miao Chen
mouseIF

Sci Rep

SERCA2a ameliorates cardiomyocyte T-tubule remodeling via the calpain/JPH2 pathway to improve cardiac function in myocardial ischemia/reperfusion mice.

Authors - Shuai Wang
mouseWB

J Am Heart Assoc

Direct Cardiac Actions of the Sodium Glucose Co-Transporter 2 Inhibitor Empagliflozin Improve Myocardial Oxidative Phosphorylation and Attenuate Pressure-Overload Heart Failure.

Authors - Xuan Li