CoraLite® Plus 488-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-30000

Synonyms

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin

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Validation Data Gallery

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  FC experiment of Jurkat using CL488-30000

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-KI67 (CL488-27309) (red),and 0.2 ug Control CoraLite® Plus 488 Rabbit IgG (CL488-30000) (Solid line). Blank (Dotted line). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: Jurkat cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-30000 targets Rabbit IgG control in FC applications and shows reactivity with samples.

• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: Rabbit IgG control
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 488 nm / 515 nm
• Form: Liquid
• Purification Method: N/A
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.