Rad21 antibody (mAb) (Clone 52A311)

Host / Isotype

Mouse / IgG

Reactivity

Human, Mouse

Applications

ICC, IF, WB

CloneNo.

52A311

Cat No : 39383,39384

Synonyms

HR21, SCC1, MCD1, NXP1, cohesin, RAD21, DNA damage, rad-21, rad 21, Clone 52A311, mAb, monoclonal, hHR21, antibody, antibodies, sample

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Validation Data Gallery

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  Rad21 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with Rad21 antibody at a 0.5 ug/ml dilution.

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  Rad21 antibody (mAb) tested by Western blot. HeLa nuclear extract (30 ug per lane) was probed with Rad21 mAb (Clone 52A311) (1 ug/ml).

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Product Information

• Tested Applications: ICC, IF, WB

  Applications Validated by Active Motif: ICC/IF: 0.5 ug/ml dilution WB: 0.5 - 2 ug/ml dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91245.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: This Rad21 antibody was raised against recombinant protein corresponding to the N-terminal half of mouse Rad21.
• Full Name: Rad21 antibody (mAb) (Clone 52A311)
• Synonyms: HR21, SCC1, MCD1, NXP1, cohesin, RAD21, DNA damage, rad-21, rad 21, Clone 52A311, mAb, monoclonal, hHR21, antibody, antibodies, sample
• Molecular weight: 100 kDa
• GenBank accession number: NP_033035
• RRID: AB_2688003
• Purification Method: Protein G Chromatography
• Buffer: Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Rad21 Clone 52A311 (HR21, MCD1, NXP1, hHR21) is highly similar to the gene product of S. pombe Rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. Rad21 is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of Rad21 with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.