Rad51 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
ICC, IF
Cat No : 39194,39195 39194
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
ICC, IF
Applications Validated by Active Motif: ICC/IF: 1 - 5 ug/ml dilution |
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This Rad51 antibody was raised against full-length human Rad51 expressed in E. coli. |
| Full Name | Rad51 antibody (pAb) |
| Synonyms | Rad51, Rad-51, Rad 51, pAb, polyclonal, BRCC5, RecA, antibody, antibodies, sample |
| Molecular weight | 37 kDa |
| GenBank accession number | NP_002866 |
| RRID | AB_2614951 |
| Purification Method | Protein A Chromatography |
| Buffer | PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Rad51 (BRCC5, RecA) is known to be involved in the homologous recombination and repair of DNA. Rad51 can interact with the ssDNA-binding protein RPA and Rad52, and it is thought to play roles in homologous pairing and strand transfer of DNA. Rad51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.