RanBP2 Polyclonal antibody

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  WB analysis using 27606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 27606-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 27606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RanBP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27606-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

358 kDa nucleoporin, ANE1, E3 SUMO protein ligase RanBP2, Nucleoporin Nup358, NUP358

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Ramos cells, Y79 cells
• Positive IHC detected in: rat liver tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

27606-1-AP targets RanBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RanBP2 fusion protein Ag26370
• Full Name: RAN binding protein 2
• Calculated Molecular Weight: 358 kDa
• Observed Molecular Weight: 358 kDa
• GenBank Accession Number: NM_006267
• Gene Symbol: RANBP2
• Gene ID (NCBI): 5903
• RRID: AB_2880923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49792
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RanBP2 (Ran-binding protein 2) is also named as p270 and NUP358, and belongs to the RanBP2 E3 ligase family. RanBP2 is E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PMID:11792325, PMID:22194619). RanBP2 involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PMID:7775481). It is component of the nuclear export pathway (PMID:10078529). RanBP2 recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PMID:20386726).

Publications

Species	Application	Title
human	WB	J Mol Biol; An Insect Viral Protein Disrupts Stress Granule Formation in Mammalian Cells; Authors - Jibin Sadasivan
human	WB,IHC	Front Oncol; YTHDF1 Aggravates the Progression of Cervical Cancer Through m6A-Mediated Up-Regulation of RANBP2.; Authors - Haocheng Wang ; KD Validated
human	WB	Nat Phys; Structural anisotropy results in mechano-directional transport of proteins across nuclear pores; Authors - Fani Panagaki ; KD Validated