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Recombinant Rat CD44 protein (rFc Tag) (HPLC verified)

Species

Rat

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg1458

Validation Data Gallery

  • Purity of Recombinant Rat CD44 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat CD44 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Rat CD44 was greater than 90% as determined by SEC-HPLC.

    The purity of Rat CD44 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat CD44 protein Gln22-Glu271 (Accession# O70509) with a rabbit IgG Fc tag at the C-terminus.
GeneID 25406
Accession O70509
PredictedSize 53.3 kDa
SDS-PAGE 62-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression (PMID: 29747682). CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis (PMID: 25941526). CD44 serves as an activation marker distinguishing memory and effector T cells from their naive counterparts. It also plays a role in early T cell signaling events, enhancing T cell receptor signaling (PMID: 26172046). This protein exists in multiple forms generated by alternative RNA splicing and extensive post-translational modifications.

References:

1. Chen C. et al. (2018). J Hematol Oncol. 11(1):64.
2. Gutjahr JC. et al. (2015). Front Immunol. 6:177.
3. Schumann J. et al. (2015). PLoS One. 24;10(11):e0143986.