• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Rat CXCL10/IP-10 protein (mFc Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg3207

Validation Data Gallery

  • Purity of Recombinant Rat CXCL10 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat CXCL10 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat CXCL10 protein Ile22-Pro98 (Accession# P48973) with a mouse IgG Fc tag at the C-terminus.
GeneID 245920
Accession P48973
PredictedSize 35.3 kDa
SDS-PAGE 30 kDa and 38-40kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CXCL10, known as IP-10 (Interferon-gamma inducible Protein 10 kDa), belongs to the ELR-CXC subfamily of chemokines. CXCL10 binds to the CXCR3 receptor and triggers chemotaxis, cell growth as well as apoptosis. CXCL10 also modulates immune responses by recruiting inflammatory cells to the sites of inflammation. CXCL10 can be secreted by various types of cells, including immune cells such as T lymphocytes, neutrophils, eosinophils and monocytes. It can also be secreted by stromal cells, including thyroid cells, splenocytes, endothelial cells, fibroblasts, and keratinocytes. Interestingly, CXCL10 is not only secreted by macrophages but also activates and attracts macrophages into tissues, and thus CXCL10 substantially controls the inflammatory response as well as the tissue and matrix homeostasis.

References:

1.Elemam NM, et al. (2022) Viruses. 14(11):2445.
2.Qiao X, et al. (2022) Int J Med Sci. 19(14):2058-2070.
3.Hirani DV, et al. (2023) Inflamm Regen. 43(1):52.