Recombinant Rat Lumican protein (N-rFc)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3410

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Validation Data Gallery

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <1.0 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat Lumican protein Gln19-Asn338 (Accession# P51886) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 81682
• Accession: P51886
• PredictedSize: 63.7 kDa
• Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Lumican is a member of the small leucine-rich proteoglycan (SLRP) family whose expression involves many organ/tissue systems, e.g., cornea, sclera, aorta, cartilage, liver, skeletal muscle, kidney, pancreas, brain, placenta, lung, etc. Lumican is an ECM matrikine that regulates multiple cellular activities and also has anti-MMP activity, anti-tumor activity, and promotes corneal epithelial wound healing, regulating gene expression and maintaining corneal homeostasis. Furthermore, Lumican is expressed in various cancer tissues and is reported to have a positive or negative correlation with tumor progression.

References:

1.Giatagana EM. et al. (2021) Biomolecules. 11(9):1319. 
2.Tsui MC. et al. (2023) Ocul Surf. 29:388-397.
3.Smith MM and Melrose J. (2024) Int J Mol Sci. 25(5):2825.