Recombinant AKT1 protein

Expressed In

Baculovirus

Protein Species

Human

Cat No : 31511

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Validation Data Gallery

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  Recombinant AKT1 protein gel. AKT1 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

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Product Information

• Expressed In: Baculovirus
• Protein Species: Human

Contents

Full length recombinant AKT1 protein expressed in Sf9 cells and supplied in 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.

Background

AKT1 is a serine-threonine protein kinase that is activated by Platelet-Derived Growth Factor (PDGF) and PI 3-kinase signaling pathways. AKT has been shown to be a critical mediator of survival and apoptosis signaling events. Activation of AKT1 involves (and requires) phosphorylation of serine 473. AKT is also referred to as Protein Kinase B (PKB) and RAC.

Application Notes

Recombinant AKT1 is suitable for kinase assays. The molecular weight of the fusion protein is ~ 81 kDa. Specific activity: 55 pmol/μg×min ATP-KM: 5.0 μM

Protein Details

Recombinant AKT1 (accession number NP_005154.2) activated with PDK1, was expressed in Sf9 cells as an N-terminal GST-HIS6 fusion protein with a 3C cleavage site.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.