Recombinant Histone H3/H4 tetramer
Expressed In
E. coli
Protein Species
Human
Cat No : 81169,81869 81169
Validation Data Gallery
Product Information
| Expressed In | E. coli |
| Protein Species | Human |
Contents
Recombinant Histone H3/H4 Tetramer is in 10 mM Tris-HCl pH 7.4, 2 M NaCl, 1 mM EDTA, 10% glycerol, and 5 mM b-mercaptoethanol.
Background
Histone H3 and H4 are the core components of the nucleosome. H3 and H4 assemble H3/H4 tetramer, and H3/K4 tetramer combines with two H2A/H2B dimers to form histone octamer. The histone octamer is wrapped around by 146 bp DNA to assemble a nucleosome, which is the basic structural unit of chromatin in vivo.
Application Notes
Recombinant Histone H3/H4 Tetramer is suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Protein Details
Recombinant histone H3/K4 Tetramer consists of full length human histones H3 (accession number NP_003520.1) and full length human histone H4 (accession number NP_003539.1). Histone H3 and H4 were expressed in E. coli cells. Recombinant histone H3/H4 Tetramer was assembled by dialysis and purified by gel filtration. The molecular weight of the Histone H3/H4 Tetramer is 53 kDa.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.