• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant STAT3 protein

Expressed In

Baculovirus

Protein Species

Human

Activ Motif logo

Cat No : 81095,81895 81095

Validation Data Gallery

  • Recombinant STAT3 protein gel 10% SDS-PAGE Coomassie staining MW: 89.2 kDa Purity: >90%

    Recombinant STAT3 protein gel 10% SDS-PAGE Coomassie staining MW: 89.2 kDa Purity: >90%

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Recombinant STAT3 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.

Background

STAT3 (Signal Transducer And Activator Of Transcription 3), also known as APRF (Acute-Phase Response Factor) or ADMIO, HIES, is a signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, STAT3 can recruit coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. Deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. STAT3 can bind to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. It also acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells. It is involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant human STAT3 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_644805.1) with an N-terminal FLAG tag. The molecular weight of the protein is 89.2 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.