Red fluorescent protein (RFP611) Polyclonal antibody

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  WB analysis of Transfected HEK-293 using 28063-1-AP

  WB result of Red fluorescent protein antibody (28063-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and Red fluorescent protein overexpressed Transfected HEK-293 cells.

Red fluorescent protein (RFP611) Polyclonal Antibody for WB, ELISA

Cat No. 28063-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

parasicyonis actinostoloides, recombinant protein

Applications

WB, ELISA

Synonyms

Red fluorescent protein, RFP, Red fluorescent protein eqFP611, GFP-like chromoprotein, eqFP611

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Tested Applications

• Positive WB detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28063-1-AP targets Red fluorescent protein (RFP611) in WB, ELISA applications and shows reactivity with parasicyonis actinostoloides, recombinant protein samples.

• Tested Reactivity: parasicyonis actinostoloides, recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Red fluorescent protein (RFP611) fusion protein Ag27893
• Full Name: Red fluorescent protein (RFP611)
• Calculated Molecular Weight: 26 kDa
• RRID: AB_2918144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8ISF8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a rabbit polyclonal antibody raised against RFP from Entacmaea quadricolor (eqFP611).