Repetin Polyclonal antibody

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  WB analysis using 25968-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25968-1-AP (Repetin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Repetin Polyclonal Antibody for WB, ELISA

Cat No. 25968-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RPTN

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Tested Applications

• Positive WB detected in: A375 cells, A431 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25968-1-AP targets Repetin in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Repetin fusion protein Ag23257
• Full Name: repetin
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC112216
• Gene Symbol: RPTN
• Gene ID (NCBI): 126638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6XPR3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Repetin (also known as RPTN), is a member of the "fused" gene family and localized in the epidermal differentiation complex on chromosome 1q21. Repetin contains 2 N-terminal EF hand motifs, a central domain containing 28 repeats of the consensus sequence QxDRQGQSSHYG, and a glutamine- and arginine-rich C-terminal domain(PMID: 15854042). Repetin is an extracellular epidermal matrix protein involved in forming keratinocyte envelope. Repetin is expressed in the epidermis and at high levels in eccrine sweat glands, the inner sheaths of hair roots, and the filiform papilli of the tongue(PMID: 20448178).