|Positive WB detected in||mouse lung tissue, human lung tissue, mouse small intestine tissue, rat skin tissue|
|Positive IHC detected in||human lung cancer tissue, mouse lung tissue, human skin tissue, human pancreas cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:1000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11250-1-AP targets S100A10 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100A10 fusion protein Ag1779|
|Full Name||S100 calcium binding protein A10|
|Calculated molecular weight||11 kDa|
|Observed molecular weight||11 kDa|
|GenBank accession number||BC015973|
|Gene ID (NCBI)||6281|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.
|Product Specific Protocols|
|WB protocol for S100A10 antibody 11250-1-AP||Download protocol|
|IHC protocol for S100A10 antibody 11250-1-AP||Download protocol|
|IF protocol for S100A10 antibody 11250-1-AP||Download protocol|
|FC protocol for S100A10 antibody 11250-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Silencing of circIgf1r plays a protective role in neuronal injury via regulating astrocyte polarization during epilepsy.
Label-free quantification proteomics reveals novel calcium binding proteins in matrix vesicles isolated from mineralizing Saos-2 cells.
Proteomic analysis of human periodontal ligament cells under hypoxia.
Down-regulation effects of IFN-α on p11, 5-htr1b and 5-HTR4 protein levels were affected by NH4CL or MG132 treatment in SH-sy5y cells.
J Biol Chem
Feedback regulation of endothelial cell surface plasmin generation by PKC-dependent phosphorylation of annexin A2.
S100A10, a Novel Biomarker in Pancreatic Ductal Adenocarcinoma.