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S100A10 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 11250-1-AP

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Con: 44 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

S100A10 Antibody WB mouse lung tissue 11250-1-AP

mouse lung tissue were subjected to SDS PAGE followed by western blot with 11250-1-AP(S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

S100A10 Antibody IHC human lung cancer tissue 11250-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP( S100A10 Antibody) at dilution of 1:400 (under 10x lens).

S100A10 Antibody IHC human lung cancer tissue 11250-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP( S100A10 Antibody) at dilution of 1:400 (under 40x lens).

S100A10 Antibody IF HeLa cells 11250-1-AP

Immunofluorescent analysis of HeLa cells using 11250-1-AP(S100A10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

S100A10 Antibody FC HeLa cells 11250-1-AP

1X10^6 HeLa cells were stained with 0.2ug S100A10 antibody (11250-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse lung tissue, human lung tissue, mouse small intestine tissue, rat skin tissue
Positive IHC detected in:
human lung cancer tissue, mouse lung tissue, human skin tissue, human pancreas cancer tissue, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:1000
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
S100A10 fusion protein Ag1779
Full name:
S100 calcium binding protein A10
Calculated molecular weight:
11 kDa
Observed molecular weight:
11 kDa
GenBank accession number:
BC015973
Gene ID (NCBI):
6281
Gene symbol
S100A10
Synonyms
42C, ANX2L, ANX2LG, CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

Background

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.


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