S100 Beta Monoclonal antibody

S100 Beta Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G10F4

Cat no : 66616-1-Ig

Synonyms

NEF, Protein S100 B, S 100 protein beta chain, S 100 protein subunit beta, S100, S100 calcium binding protein B, S100B, S100beta



Tested Applications

Positive WB detected inrat brain tissue, C6 cells, pig brain tissue
Positive IHC detected inhuman malignant melanoma tissue, human appendicitis tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66616-1-Ig targets S100 Beta in WB, IHC, IF,ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Reactivity Human, pig, rat
Cited Reactivity mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100 Beta fusion protein Ag7440
Full Name S100 calcium binding protein B
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC001766
Gene symbol S100B
Gene ID (NCBI) 6285
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new bomarker for mood disorders.

Protocols

Product Specific Protocols
WB protocol for S100 Beta antibody 66616-1-IgDownload protocol
IHC protocol for S100 Beta antibody 66616-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF,WB

Neurochem Res

Resistin-Inhibited Neural Stem Cell-Derived Astrocyte Differentiation Contributes to Permeability Destruction of the Blood-Brain Barrier.

Authors - Liu Xiaoying
ratIF

Front Neurosci

Nogo-C Inhibits Peripheral Nerve Regeneration by Regulating Schwann Cell Apoptosis and Dedifferentiation.

Authors - Bo Jia
mouseWB

Exp Neurol

Interaction of DCF1 with ATP1B1 induces impairment in astrocyte structural plasticity via the P38 signaling pathway.

Authors - Jiao Wang