S100 Beta Monoclonal antibody

S100 Beta Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, pig, rat and More (1)







Cat no : 66616-1-Ig


NEF, Protein S100 B, S 100 protein beta chain, S 100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta

Tested Applications

Positive WB detected inrat brain tissue, C6 cells, pig brain tissue
Positive IHC detected inhuman malignant melanoma tissue, human appendicitis tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66616-1-Ig targets S100 Beta in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100 Beta fusion protein Ag7440
Full Name S100 calcium binding protein B
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC001766
Gene Symbol S100 Beta
Gene ID (NCBI) 6285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.


Product Specific Protocols
WB protocol for S100 Beta antibody 66616-1-IgDownload protocol
IHC protocol for S100 Beta antibody 66616-1-IgDownload protocol
IF protocol for S100 Beta antibody 66616-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Acta Neuropathol Commun

Repetitive mild TBI causes pTau aggregation in nigra without altering preexisting fibril induced Parkinson’s-like pathology burden

Authors - Vedad Delic

Cell Mol Life Sci

Adtrp regulates thermogenic activity of adipose tissue via mediating the secretion of S100b.

Authors - Peng Li

Exp Neurol

Interaction of DCF1 with ATP1B1 induces impairment in astrocyte structural plasticity via the P38 signaling pathway.

Authors - Jiao Wang

Front Pharmacol

Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway.

Authors - Binbin Hu

ACS Omega

Platelet-Rich Plasma Gel-Loaded Collagen/Chitosan Composite Film Accelerated Rat Sciatic Nerve Injury Repair

Authors - Bo Yuan

Front Neurosci

Nogo-C Inhibits Peripheral Nerve Regeneration by Regulating Schwann Cell Apoptosis and Dedifferentiation.

Authors - Bo Jia


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Reyes (Verified Customer) (07-02-2024)

s100b (in green) works in human brain FFPE tissue, but it does not yield the best results. (It colocalizes in a small way with NeuN in red).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
S100 Beta Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:66616-1-Ig)

Gabriele (Verified Customer) (06-06-2024)

The antibody works well, staining positively the hiPSC-derived astrocytes in green (1:200). It can colocalize with GFAP (in red). Nuclei are stained with hoechst (in blue)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: hiPSC-derived Astrocytes
S100 Beta Antibody Immunofluorescence validation (1:200 dilution) in hiPSC-derived Astrocytes (Cat no:66616-1-Ig)

Clarisse (Verified Customer) (08-08-2022)

This antibody works very well as an astrocyte marker. In the image: in green mouse anti-S100B (1:500 - 66616-1-Ig) and in magenta rabbit anti-S100B (1:500 - 15146-1-AP). The rabbit antibody has a better performance.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human cerebral organoids
S100 Beta Antibody Immunofluorescence validation (1:500 dilution) in Human cerebral organoids (Cat no:66616-1-Ig)