Validation Data Gallery
|Positive WB detected in||rat brain tissue, C6 cells, pig brain tissue|
|Positive IHC detected in||human malignant melanoma tissue, human appendicitis tissue, human brain tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66616-1-Ig targets S100 Beta in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
|Tested Reactivity||Human, mouse, pig, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||S100 Beta fusion protein Ag7440|
|Full Name||S100 calcium binding protein B|
|Calculated molecular weight||11 kDa|
|Observed molecular weight||11 kDa|
|GenBank accession number||BC001766|
|Gene ID (NCBI)||6285|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.
Resistin-Inhibited Neural Stem Cell-Derived Astrocyte Differentiation Contributes to Permeability Destruction of the Blood-Brain Barrier.
High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.
Nogo-C Inhibits Peripheral Nerve Regeneration by Regulating Schwann Cell Apoptosis and Dedifferentiation.
Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway.
Interaction of DCF1 with ATP1B1 induces impairment in astrocyte structural plasticity via the P38 signaling pathway.
Cell Mol Life Sci
Adtrp regulates thermogenic activity of adipose tissue via mediating the secretion of S100b.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Clarisse (Verified Customer) (08-08-2022)
This antibody works very well as an astrocyte marker. In the image: in green mouse anti-S100B (1:500 - 66616-1-Ig) and in magenta rabbit anti-S100B (1:500 - 15146-1-AP). The rabbit antibody has a better performance.