SAE1 Polyclonal antibody
SAE1 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, ELISA
Cat no : 10229-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, A549 cells, COLO 320 cells, HEK-293 cells, HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10229-1-AP targets SAE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SAE1 fusion protein Ag0275|
|Full Name||SUMO1 activating enzyme subunit 1|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||38 kDa|
|GenBank accession number||BC000344|
|Gene ID (NCBI)||10055|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).
|Product Specific Protocols|
|WB protocol for SAE1 antibody 10229-1-AP||Download protocol|
|IHC protocol for SAE1 antibody 10229-1-AP||Download protocol|
|IF protocol for SAE1 antibody 10229-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.
Open Med (Wars)
Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation.
SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation
TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.