SAE1 Polyclonal antibody

SAE1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10229-1-AP

Synonyms

AOS1, FLJ3091, HSPC140, SAE1, SUA1, UBLE1A



Tested Applications

Positive WB detected inA431 cells, A549 cells, COLO 320 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

10229-1-AP targets SAE1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAE1 fusion protein Ag0275
Full Name SUMO1 activating enzyme subunit 1
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000344
Gene symbol SAE1
Gene ID (NCBI) 10055
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

Protocols

Product Specific Protocols
WB protocol for SAE1 antibody 10229-1-APDownload protocol
IHC protocol for SAE1 antibody 10229-1-APDownload protocol
IF protocol for SAE1 antibody 10229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.

Authors - Xiancai Ma
mouseWB

Curr Biol

Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.

Authors - Dong Chen