SAE1 Polyclonal antibody

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  WB analysis of A431 using 10229-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 10229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10229-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10229-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SAE1 antibody (10229-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SAE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10229-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SUMO-activating enzyme subunit 1, N-terminally processed, SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, HEK-293 cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

10229-1-AP targets SAE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAE1 fusion protein Ag0275
• Full Name: SUMO1 activating enzyme subunit 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000344
• Gene Symbol: SAE1
• Gene ID (NCBI): 10055
• RRID: AB_2182917
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBE0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

Publications

Species	Application	Title
mouse	WB	Curr Biol; Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.; Authors - Dong Chen
human	IHC	Open Med (Wars); Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation.; Authors - Yang Liu
human	WB	Nat Commun; SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation; Authors - Lingjie Yan
human	IHC,WB	Curr Issues Mol Biol; SUMO-Activating Enzyme Subunit 1 Is Associated with Poor Prognosis, Tumor Progression, and Radio-Resistance in Colorectal Cancer; Authors - Yueh-Jung Wu
human	WB	Elife; TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.; Authors - Xiancai Ma