Validation Data Gallery
|Positive IHC detected in||human liver tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23393-1-AP is SCD Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SCD fusion protein Ag13456|
|Full Name||stearoyl-CoA desaturase (delta-9-desaturase)|
|Calculated molecular weight||355 aa, 41 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC005807|
|Gene ID (NCBI)||6319|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SCD (stearoyl-CoA desaturase) is a 37-45 kDa (PMID:10946019) microsomal fatty acid monodesaturase, also commonly known as 9-desaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids. Five SCD genes (SCD1, SCD2, SCD3,SCD4 and SCD5) have been identiﬁed and characterized in mice and SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively, SCD3 is expressed exclusively in skin, whereas SCD4 is expressed predominantly in the heart. SCD1and SCD5 has been the only human SCD gene identified and characterized todate(PMID:15907797 ). The formation of homodimers and oligomers is an intrinsic property of SCD proteins, which may play an important role in regulating the half-life of the SCDenzymes, thus representing a novel regulatory mechanism for SCD enzymes, in addition to the transcriptional and post-translational regulations. A 32-kDa SCD1 degradation product identified may be caused by a major cleavage site at the C-terminus, thus representing a novel degradation product(PMID:15610069).
Downregulation of PDK4 Increases Lipogenesis and Associates with Poor Prognosis in Hepatocellular Carcinoma.
Biochem Biophys Res Commun
Inhibition of fatty acid synthesis arrests colorectal neoplasm growth and metastasis: Anti-cancer therapeutical effects of natural cyclopeptide RA-XII.
A lipid-free and insulin-supplemented medium supports De Novo fatty acid synthesis gene activation in melanoma cells.
Biochim Biophys Acta
Brg1 regulates pro-lipogenic transcription by modulating SREBP activity in hepatocytes.
Evid Based Complement Alternat Med
Dendrobium officinale Regulates Fatty Acid Metabolism to Ameliorate Liver Lipid Accumulation in NAFLD Mice.
Pharmacol Res Perspect
Dexmedetomidine ameliorates high-fat diet-induced nonalcoholic fatty liver disease by targeting SCD1 in obesity mice.