SCN4B Polyclonal antibody

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  WB analysis using 30916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30916-1-AP (SCN4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SCN4B Polyclonal Antibody for WB, ELISA

Cat No. 30916-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Sodium channel subunit beta-4, Sodium channel subunit beta 4

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Tested Applications

• Positive WB detected in: HepG2 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30916-1-AP targets SCN4B in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCN4B fusion protein Ag34123
• Full Name: sodium channel, voltage-gated, type IV, beta
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_174934.3
• Gene Symbol: SCN4B
• Gene ID (NCBI): 6330
• RRID: AB_3669784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8IWT1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in the SCN4B gene are a cause of long QT syndrome type 10 (LQT10).