OXCT1 Recombinant antibody

OXCT1 Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4K12

Cat no : 81011-1-RR

Synonyms

3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse skeletal muscle, mouse brain, rat brain, mouse heart, rat heart
Positive IF detected inSKOV-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

81011-1-RR targets OXCT1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OXCT1 fusion protein Ag2818
Full Name 3-oxoacid CoA transferase 1
Calculated molecular weight 520 aa, 56 kDa
Observed molecular weight56 kDa
GenBank accession numberBC009001
Gene symbol OXCT1
Gene ID (NCBI) 5019
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Protocols

Product Specific Protocols
WB protocol for OXCT1 antibody 81011-1-RRDownload protocol
IF protocol for OXCT1 antibody 81011-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols