CD138/Syndecan-1 Polyclonal antibody
CD138/Syndecan-1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 10593-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
10593-1-AP targets CD138/Syndecan-1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD138/Syndecan-1 fusion protein Ag0823 |
| Full Name | syndecan 1 |
| Calculated molecular weight | 32.5 kDa |
| Observed molecular weight | 60-100 kDa |
| GenBank accession number | BC008765 |
| Gene symbol | SDC1 |
| Gene ID (NCBI) | 6382 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD138/Syndecan-1 antibody 10593-1-AP | Download protocol |
| IHC protocol for CD138/Syndecan-1 antibody 10593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immunology IL-33 induces production of autoantibody against autologous respiratory epithelial cells: a potential mechanism for the pathogenesis of COPD. | ||
Cell Death Dis CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma. | ||
Biomed Res Int PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma. | ||
Cell Death Discov Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma.
| ||
Pathol Oncol Res Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway. |
