CD138/Syndecan-1 Polyclonal antibody

CD138/Syndecan-1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10593-1-AP

Synonyms

CD138, SDC, SDC1, SYND1, syndecan, syndecan 1



Tested Applications

Positive WB detected inRaji cells, HeLa cells
Positive IHC detected inhuman tonsillitis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Sample-dependent, check data in validation data gallery

Product Information

10593-1-AP targets CD138/Syndecan-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD138/Syndecan-1 fusion protein Ag0823
Full Name syndecan 1
Calculated molecular weight 32.5 kDa
Observed molecular weight 60-100 kDa
GenBank accession numberBC008765
Gene symbol SDC1
Gene ID (NCBI) 6382
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.

Protocols

Product Specific Protocols
WB protocol for CD138/Syndecan-1 antibody 10593-1-APDownload protocol
IHC protocol for CD138/Syndecan-1 antibody 10593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Immunology

IL-33 induces production of autoantibody against autologous respiratory epithelial cells: a potential mechanism for the pathogenesis of COPD.

Authors - Qin Li
humanIF

Cell Death Dis

CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma.

Authors - Ruyi Xu
humanIHC

Biomed Res Int

PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma.

Authors - Hua Bai
humanIHC

J Leukoc Biol

PMID: 30024656

Authors - Yao Yao
humanIP

Pathol Oncol Res

Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway.

Authors - Ding Weifeng W

J Virol

Neglected but Important Role of Apolipoprotein E Exchange in Hepatitis C Virus Infection.

Authors - Zaili Yang