Validation Data Gallery
|Positive WB detected in||A549 cells, MCF-7 cells, rat kidney tissue, Jurkat cells, mouse kidney tissue, L02 cells, SH-SY5Y cells, mouse lung tissue, rat lung tissue|
|Positive IP detected in||mouse lung tissue, A549 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-12 cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10886-1-AP targets Syndecan-3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Syndecan-3 fusion protein Ag1317|
|Full Name||syndecan 3|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||60-70 kDa|
|GenBank accession number||BC013974|
|Gene ID (NCBI)||9672|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
|Product Specific Protocols|
|WB protocol for Syndecan-3 antibody 10886-1-AP||Download protocol|
|IHC protocol for Syndecan-3 antibody 10886-1-AP||Download protocol|
|IF protocol for Syndecan-3 antibody 10886-1-AP||Download protocol|
|IP protocol for Syndecan-3 antibody 10886-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Am J Pathol
Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.
Am J Pathol
No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.
J Cell Sci
Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.
Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.
Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3
Int J Mol Sci
GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (02-28-2019)
Tissue was fixed in PFA with tris-HCl antigen retrieval ph=6. Co-localisation with microglia based on known markers (not shown).