Syndecan-3 Polyclonal antibody

Syndecan-3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10886-1-AP


KIAA0468, N syndecan, SDC3, SDCN, SYND3, syndecan 3, Syndecan3, Syndecan-3

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, rat kidney tissue, Jurkat cells, mouse kidney tissue, L02 cells, SH-SY5Y cells, mouse lung tissue, rat lung tissue
Positive IP detected inmouse lung tissue, A549 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, MCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10886-1-AP targets Syndecan-3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syndecan-3 fusion protein Ag1317
Full Name syndecan 3
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC013974
Gene Symbol SDC3
Gene ID (NCBI) 9672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.


Product Specific Protocols
WB protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IHC protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IF protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IP protocol for Syndecan-3 antibody 10886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Am J Pathol

Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.

Authors - van Oosterwijk Jolieke G JG

Am J Pathol

No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.

Authors - Reijnders Christianne M A CM

J Cell Sci

Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.

Authors - Yu-Ja Huang

BMC Cancer

Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.

Authors - Wu Zheng-Sheng ZS

Medicine (Baltimore)

Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3

Authors - Guozhi Yang

Int J Mol Sci

GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway

Authors - Apostolos Mikroulis


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:2000 in 1X TBST incubated overnight at 4 degree C. Syndecan-3 appeared at 65 kDa.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: WESTERN BLOT (1:2000), IMMUNOFLUORESCENCE (1:100)
  • Cell Tissue Type: Mouse liver tissue

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with tris-HCl antigen retrieval ph=6. Co-localisation with microglia based on known markers (not shown).

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish retina (Microglia) at 3dpf
Syndecan-3 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Microglia) at 3dpf (Cat no:10886-1-AP)