Syndecan-3 Polyclonal antibody

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  WB analysis using 10886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10886-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 10886-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10886-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10886-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10886-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10886-1-AP

  IP result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:1000) with mouse lung tissue lysate 4000ug.

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  IP experiment of A549 using 10886-1-AP

  IP result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:600) with A549 cells lysate 2800ug.

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  IHC staining of human colon cancer using 10886-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10886-1-AP (Syndecan-3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10886-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10886-1-AP (Syndecan-3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 10886-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Syndecan-3 antibody (10886-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10886-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SDC3 antibody 10886-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Syndecan-3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10886-1-AP

Publications(6)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SDC3, syndecan 3, SYND3, SDCN, N syndecan

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, rat kidney tissue, Jurkat cells, mouse kidney tissue, L02 cells, SH-SY5Y cells, mouse lung tissue, rat lung tissue
• Positive IP detected in: mouse lung tissue, A549 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-12 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

10886-1-AP targets Syndecan-3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syndecan-3 fusion protein Ag1317
• Full Name: syndecan 3
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC013974
• Gene Symbol: Syndecan-3
• Gene ID (NCBI): 9672
• RRID: AB_2182999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75056
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.

Publications

Species	Application	Title
human	IHC	Am J Pathol; Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.; Authors - van Oosterwijk Jolieke G JG
human	IHC	Am J Pathol; No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.; Authors - Reijnders Christianne M A CM
human	WB	J Cell Sci; Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.; Authors - Yu-Ja Huang
human	IHC	BMC Cancer; Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.; Authors - Wu Zheng-Sheng ZS
human	IHC	Medicine (Baltimore); Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3; Authors - Guozhi Yang
mouse	IF	Int J Mol Sci; GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway; Authors - Apostolos Mikroulis

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:2000 in 1X TBST incubated overnight at 4 degree C. Syndecan-3 appeared at 65 kDa.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WESTERN BLOT (1:2000), IMMUNOFLUORESCENCE (1:100)
• Cell Tissue Type: Mouse liver tissue

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with tris-HCl antigen retrieval ph=6. Co-localisation with microglia based on known markers (not shown).

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina (Microglia) at 3dpf

Syndecan-3 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Microglia) at 3dpf (Cat no:10886-1-AP)