Syndecan-3 Polyclonal antibody

Syndecan-3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10886-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

SDC3, syndecan 3, SYND3, SDCN, N syndecan

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, rat kidney tissue, Jurkat cells, mouse kidney tissue, L02 cells, SH-SY5Y cells, mouse lung tissue, rat lung tissue
Positive IP detected inmouse lung tissue, A549 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10886-1-AP targets Syndecan-3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syndecan-3 fusion protein Ag1317 Predict reactive species
Full Name syndecan 3
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC013974
Gene Symbol Syndecan-3
Gene ID (NCBI) 9672
RRIDAB_2182999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75056
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.

Protocols

Product Specific Protocols
WB protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IHC protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IF protocol for Syndecan-3 antibody 10886-1-APDownload protocol
IP protocol for Syndecan-3 antibody 10886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
HumanWB

Nucleic Acids Res

Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease.

Authors - Kana Asano
humanIHC

Am J Pathol

No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.

Authors - Reijnders Christianne M A CM
humanIHC

Am J Pathol

Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.

Authors - van Oosterwijk Jolieke G JG
humanWB

J Cell Sci

Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.

Authors - Yu-Ja Huang
humanIHC

BMC Cancer

Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.

Authors - Wu Zheng-Sheng ZS
humanIHC

Medicine (Baltimore)

Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3

Authors - Guozhi Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:2000 in 1X TBST incubated overnight at 4 degree C. Syndecan-3 appeared at 65 kDa.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: WESTERN BLOT (1:2000), IMMUNOFLUORESCENCE (1:100)
  • Cell Tissue Type: Mouse liver tissue
FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with tris-HCl antigen retrieval ph=6. Co-localisation with microglia based on known markers (not shown).

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish retina (Microglia) at 3dpf
Syndecan-3 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Microglia) at 3dpf (Cat no:10886-1-AP)
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