|Positive WB detected in||A549 cells, human placenta tissue, HeLa cells, MCF-7 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22399-1-AP is Syntenin-1 Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Syntenin-1 fusion protein Ag18078|
|Full Name||syndecan binding protein (syntenin)|
|Calculated molecular weight||298 aa, 32 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC113674|
|Gene ID (NCBI)||6386|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.
|Product Specific Protocols|
|WB protocol for Syntenin-1 antibody 22399-1-AP||Download protocol|
|IHC protocol for Syntenin-1 antibody 22399-1-AP||Download protocol|
|IF protocol for Syntenin-1 antibody 22399-1-AP||Download protocol|
|IP protocol for Syntenin-1 antibody 22399-1-AP||Download protocol|
|FC protocol for Syntenin-1 antibody 22399-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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