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  • KD/KO Validated

Syntenin-1 Polyclonal antibody

Syntenin-1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 22399-1-AP

Synonyms

MDA 9, MDA9, Scaffold protein Pbp1, SDCBP, ST1, SYCL, Syndecan binding protein 1, Syntenin-1, TACIP18



Tested Applications

Positive WB detected inA549 cells, human placenta tissue, HeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22399-1-AP is Syntenin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntenin-1 fusion protein Ag18078
Full Name syndecan binding protein (syntenin)
Calculated molecular weight 298 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC113674
Gene symbol SDCBP
Gene ID (NCBI) 6386
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.

Protocols

Product Specific Protocols
WB protocol for Syntenin-1 antibody 22399-1-APDownload protocol
IHC protocol for Syntenin-1 antibody 22399-1-APDownload protocol
IF protocol for Syntenin-1 antibody 22399-1-APDownload protocol
IP protocol for Syntenin-1 antibody 22399-1-APDownload protocol
FC protocol for Syntenin-1 antibody 22399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

A taspine derivative supresses Caco-2 cell growth by competitively targeting EphrinB2 and regulating its pathway.

Authors - Bingling Dai
humanWB

Phytomedicine

Berberine inhibits the proliferation and migration of breast cancer ZR-75-30 cells by targeting Ephrin-B2.

Authors - Weina Ma
humanWB

Oncol Rep

Downregulation of exosomal miR‑1273a increases cisplatin resistance of non‑small cell lung cancer by upregulating the expression of syndecan binding protein.

Authors - Xiaolong Zhao
humanWB

Biophys J

Effects of Inhibiting VPS4 Support a General Role for ESCRTs in Extracellular Vesicle Biogenesis.

Authors - Charles E Jackson
humanWB

Cell Oncol (Dordr)

Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2.

Authors - Ningye Ma
humanWB

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
  • KO Validated