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SDHA Antibody 18 Publications

Rabbit Polyclonal| Catalog number: 14865-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

SDHA Antibody WB HEK-293 cells 14865-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14865-1-AP(SDHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

SDHA Antibody IF HepG2 cells 14865-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 14865-1-AP (SDHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

SDHA Antibody IP mouse brain tissue 14865-1-AP

IP Result of anti-SDHA (IP:14865-1-AP, 4ug; Detection:14865-1-AP 1:500) with mouse brain tissue lysate 4000ug.

SDHA Antibody IHC human liver cancer tissue 14865-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP( SDHA Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

SDHA Antibody IHC human liver cancer tissue 14865-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14865-1-AP( SDHA Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat, pig, zebrafish
Positive WB detected in:
HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human liver cancer tissue, human heart tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, mouse liver tissue, mouse heart tissue, mouse brain tissue
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SDHA fusion protein Ag6677
Full name:
succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Calculated molecular weight:
73 kDa
Observed molecular weight:
62-73 kDa
GenBank accession number:
BC001380
Gene ID (NCBI):
6389
Gene symbol
SDHA
Synonyms
FP, SDH2, SDHA, SDHF

Background

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.


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