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  • KD/KO Validated

SDHA Polyclonal antibody

SDHA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14865-1-AP

Synonyms

FP, SDH2, SDHA, SDHF



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse liver tissue, mouse heart tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14865-1-AP targets SDHA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDHA fusion protein Ag6677
Full Name succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Calculated molecular weight 73 kDa
Observed molecular weight 62-73 kDa
GenBank accession numberBC001380
Gene symbol SDHA
Gene ID (NCBI) 6389
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Protocols

Product Specific Protocols
WB protocol for SDHA antibody 14865-1-APDownload protocol
IHC protocol for SDHA antibody 14865-1-APDownload protocol
IF protocol for SDHA antibody 14865-1-APDownload protocol
IP protocol for SDHA antibody 14865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Free Radic Biol Med

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Authors - Yuanzhen Ma
zebrafishWB

Nucleic Acids Res

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Authors - Danni Chen
mouseWB

Am J Physiol Renal Physiol

The increase of core temperature affected the progression of kidney injury by repeated heat stress exposure.

Authors - Yuka Sato
pigWB

Br J Pharmacol

PMID: 30515768

Authors - Xiaoying Lian
humanWB

Nat Metab

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Authors - Shi-Ting Li
  • KD Validated
humanWB

Dis Markers

CYC1 Predicts Poor Prognosis in Patients with Breast Cancer.

Authors - Yingyan Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jessica (Verified Customer) (08-19-2019)

This antibody worked great for western blots on whole cell lysates and purified mitochondria!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Osteosarcoma cell line