Tested Applications
| Positive WB detected in | HEK-293 cells, pig brain tissue, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue, zebrafish |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14865-1-AP targets SDHA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish samples.
| Tested Reactivity | human, mouse, rat, pig, zebrafish |
| Cited Reactivity | human, mouse, rat, pig, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6677 Product name: Recombinant human SDHA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-664 aa of BC001380 Sequence: DLASRDVVSRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY Predict reactive species |
| Full Name | succinate dehydrogenase complex, subunit A, flavoprotein (Fp) |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 62-73 kDa |
| GenBank Accession Number | BC001380 |
| Gene Symbol | SDHA |
| Gene ID (NCBI) | 6389 |
| RRID | AB_11182164 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31040 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SDHA antibody 14865-1-AP | Download protocol |
| IHC protocol for SDHA antibody 14865-1-AP | Download protocol |
| IP protocol for SDHA antibody 14865-1-AP | Download protocol |
| WB protocol for SDHA antibody 14865-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SDHA antibody (Cat# 14865-1-AP) has accumulated 163 citations across journals including Science, Cell, Nature Communications, Nature Metabolism, Cell Metabolism, Molecular Cell, EMBO Journal, Nature Structural & Molecular Biology, and Cell Reports. Research fields span mitochondrial biology (TCA cycle, electron transport, mitophagy, cristae dynamics), oncology (metabolic reprogramming in liver, lung, colorectal, breast, and hematologic cancers), neurodegeneration, cardiovascular disease, immunology, metabolic disorders (obesity, diabetes), and oxidative stress/ferroptosis.
| Species | Application | Title |
|---|---|---|
Science Fumarate is a terminal electron acceptor in the mammalian electron transport chain.
| ||
Circulation Nuclear Receptor NR1D1 Regulates Abdominal Aortic Aneurysm Development by Targeting the Mitochondrial Tricarboxylic Acid Cycle Enzyme Aconitase-2 | ||
Cell Metab Ergothioneine controls mitochondrial function and exercise performance via direct activation of MPST | ||
Cell Metab AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. | ||
Cell Metab Iron-addicted colorectal cancers exploit heme-complex II axis to resist oxidative cell death. |
Reviews
What customers say
Users report generally positive results with this antibody for Western Blot across multiple cell types. One reviewer found it worked great on osteosarcoma cell lysates and purified mitochondria at 1:1000. Another achieved good results on lymphoblastoid cells at 1:10000 but noted light unspecific bands. A third user obtained acceptable readout on zebrafish material at 1/3000. Overall, the antibody performs well for WB with some variability in specificity depending on the sample type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lenie (Verified Customer) (10-30-2025) | Zebrafish material, used dilution 1/3000, acceptable read out on WB.
 |
Baptiste (Verified Customer) (05-23-2025) | Working well but have light unspecific bands
 |
Jessica (Verified Customer) (08-19-2019) | This antibody worked great for western blots on whole cell lysates and purified mitochondria!
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