|Positive WB detected in||Jurkat cells, L02 cells, HUVEC cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:800-1:3200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20894-1-AP is E-selectin /CD62E Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||E-selectin /CD62E fusion protein Ag14944|
|Full Name||selectin E|
|Calculated molecular weight||610 aa, 67 kDa|
|Observed molecular weight||67 kDa|
|GenBank accession number||BC142711|
|Gene ID (NCBI)||6401|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)
|Product Specific Protocols|
|WB protocol for E-selectin /CD62E antibody 20894-1-AP||Download protocol|
|IHC protocol for E-selectin /CD62E antibody 20894-1-AP||Download protocol|
|FC protocol for E-selectin /CD62E antibody 20894-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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