E-selectin/CD62E Polyclonal antibody

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  WB analysis using 20894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20894-1-AP (E-selectin/CD62E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 20894-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 20894-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 20894-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20894-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

E-selectin/CD62E Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20894-1-AP

Publications(46)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

E-selectin / CD62E, CD62E, E-selectin, CD62 antigen-like family member E, ELAM1

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Tested Applications

• Positive WB detected in: HUVEC cells
• Positive IHC detected in: human appendicitis tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 37 publications below
• IHC: See 3 publications below
• IF: See 9 publications below

Product Information

20894-1-AP targets E-selectin/CD62E in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E-selectin/CD62E fusion protein Ag14944
• Full Name: selectin E
• Calculated Molecular Weight: 610 aa, 67 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC142711
• Gene Symbol: CD62E
• Gene ID (NCBI): 6401
• RRID: AB_10755287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16581
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury; Authors - Xun Wu
human	WB	Sci Adv; The support of genetic evidence for cardiovascular risk induced by antineoplastic drugs.; Authors - Hui Cui
human	IF	Adv Healthc Mater; Boosting Tumor Accumulation of Phthalocyanine through Sialylation Engineering for Superior Cancer Phototherapy; Authors - Jian Li
	WB	ACS Appl Mater Interfaces; Inflammation-Driven Nanohitchhiker Enhances Postoperative Immunotherapy by Alleviating Prostaglandin E2-Mediated Immunosuppression; Authors - Yingke Liu
human	IF	Biomater Sci; An E-selectin targeting and MMP-2-responsive dextran-curcumin polymeric prodrug for targeted therapy of acute kidney injury; Authors - Jing-Bo Hu
human	WB	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yannic (Verified Customer) (01-07-2022)

Left and right band refer to markers. Many unspecific bands, blocked with 5% BSA, primary antibody over night

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HMEC1 endothelial cells

E-selectin/CD62E Antibody Western Blot validation (1:1000 dilution) in HMEC1 endothelial cells (Cat no:20894-1-AP)