SERPINA6/CBG Polyclonal antibody

SERPINA6/CBG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12010-1-AP

Synonyms

SERPINA6, Serpin A6, CBG, Transcortin, Corticosteroid-binding globulin

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Validation Data Gallery

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  WB analysis of human plasma using 12010-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12010-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12010-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12010-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 12010-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12010-1-AP (SERPINA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma tissue, A375 cells, HEK-293 cells, L02 cells
• Positive IHC detected in: human kidney tissue, human hepatocirrhosis tissue, human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12010-1-AP targets SERPINA6/CBG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERPINA6/CBG fusion protein Ag2623
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
• Calculated Molecular Weight: 405 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC058021
• Gene Symbol: SERPINA6
• Gene ID (NCBI): 866
• RRID: AB_874108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).

Publications

Species	Application	Title
mouse	WB	Food Funct; Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism; Authors - Zehua Liu
human	WB	PLoS One; Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm.; Authors - Acosta-Martin Adelina E AE