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  • KD/KO Validated

SESN3 Polyclonal antibody

SESN3 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11431-2-AP

Synonyms

SESN3, SEST3, sestrin 3



Tested Applications

Positive WB detected inHeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

11431-2-AP targets SESN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SESN3 fusion protein Ag1961
Full Name sestrin 3
Calculated molecular weight 492 aa, 57 kDa
Observed molecular weight 51-59 kDa
GenBank accession numberBC017296
Gene symbol SESN3
Gene ID (NCBI) 143686
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Protocols

Product Specific Protocols
WB protocol for SESN3 antibody 11431-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Brain Res

Silencing rno-miR-155-5p in rat temporal lobe epilepsy model reduces pathophysiological features and cell apoptosis by activating Sestrin-3.

Authors - Li-Gang Huang
  • KD Validated
mouseWB

Biochim Biophys Acta

Exercise improves glucose uptake in murine myotubes through the AMPKα2-mediated induction of Sestrins.

Authors - Tianyi Wang
humanWB

Cell Physiol Biochem

Sestrin-Mediated Inhibition of Stress-Induced Intervertebral Disc Degradation Through the Enhancement of Autophagy.

Authors - Ji Tu
mouseWB

Appl Physiol Nutr Metab

Exercise improves lipid metabolism disorders induced by high-fat diet in a SESN2/JNK- independent manner.

Authors - Tianyi Wang
humanWB

BMC Cancer

Inhibition of mTORC1 through ATF4-induced REDD1 and Sestrin2 expression by Metformin.

Authors - Se-Kyeong Jang
humanWB

PLoS One

Regulatory Effects of Sestrin 3 (SESN3) in BCR-ABL Expressing Cells.

Authors - Vakana Eliza E