SESN3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SESN3 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11431-2-AP

Synonyms

SESN3, SEST3, sestrin 3

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Validation Data Gallery

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  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Product Information

11431-2-AP targets SESN3 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SESN3 fusion protein Ag1961
• Full Name: sestrin 3
• Calculated molecular weight: 492 aa, 57 kDa
• Observed molecular weight: 51-59 kDa
• GenBank accession number: BC017296
• Gene symbol: SESN3
• Gene ID (NCBI): 143686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Publications

Species	Application	Title
human,rat	WB	Brain Res; Silencing rno-miR-155-5p in rat temporal lobe epilepsy model reduces pathophysiological features and cell apoptosis by activating Sestrin-3.; Authors - Li-Gang Huang ; KD Validated
mouse	WB	Biochim Biophys Acta; Exercise improves glucose uptake in murine myotubes through the AMPKα2-mediated induction of Sestrins.; Authors - Tianyi Wang
human	WB	Cell Physiol Biochem; Sestrin-Mediated Inhibition of Stress-Induced Intervertebral Disc Degradation Through the Enhancement of Autophagy.; Authors - Ji Tu
human	WB	PLoS One; Regulatory Effects of Sestrin 3 (SESN3) in BCR-ABL Expressing Cells.; Authors - Vakana Eliza E
human	WB,IHC	Osteoarthritis Cartilage; Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.; Authors - T Shen
human	WB	Elife; Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation.; Authors - Veronique Nogueira ; KD Validated