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- KD/KO Validated
SESN3 Polyclonal antibody
SESN3 Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11431-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 9 publications below |
IHC | See 2 publications below |
Product Information
11431-2-AP targets SESN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SESN3 fusion protein Ag1961 |
Full Name | sestrin 3 |
Calculated molecular weight | 492 aa, 57 kDa |
Observed molecular weight | 51-59 kDa |
GenBank accession number | BC017296 |
Gene symbol | SESN3 |
Gene ID (NCBI) | 143686 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.
Protocols
Product Specific Protocols | |
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WB protocol for SESN3 antibody 11431-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Brain Res Silencing rno-miR-155-5p in rat temporal lobe epilepsy model reduces pathophysiological features and cell apoptosis by activating Sestrin-3.
| ||
Biochim Biophys Acta Exercise improves glucose uptake in murine myotubes through the AMPKα2-mediated induction of Sestrins. | ||
Cell Physiol Biochem Sestrin-Mediated Inhibition of Stress-Induced Intervertebral Disc Degradation Through the Enhancement of Autophagy. | ||
Appl Physiol Nutr Metab Exercise improves lipid metabolism disorders induced by high-fat diet in a SESN2/JNK- independent manner. | ||
BMC Cancer Inhibition of mTORC1 through ATF4-induced REDD1 and Sestrin2 expression by Metformin. | ||