SESN3 Polyclonal antibody

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• SESN3 Polyclonal antibody (11431-2-AP)

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• KD/KO Validated

SESN3 Polyclonal Antibody for WB, ELISA

Cat No. 11431-2-AP Copied!

Publications (22)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-

• 

  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11431-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11431-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 11431-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 11431-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 11431-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 11431-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 11431-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 11431-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 11431-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11431-2-AP targets SESN3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1961

  Product name: Recombinant human SESN3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-321 aa of BC017296

  Sequence: MNRGGGSPSAAANYLLCTNCRKVLRKDKRIRVSQPLTRGPSAFIPEKEVVQANTVDERTNFLVEEYSTSGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLINMHVDEFLKTGGIAEWLNGLEYVPQRLKNLNEINKLLAHRPWLITKEHIQKLVKTGENNWSLPELVHAVVLLAHYHALASFVFGSGINPERDPEISNGFRLISVNNFCVCDLANDNNIENASLSGSNFGIVDSLSELEALMERMKRLQEEREDEEASQEEMSTRFEKEKKESLFVVSGDTFHSFPHSGAFLHFFAF

• Full Name: sestrin 3
• Calculated Molecular Weight: 492 aa, 57 kDa
• Observed Molecular Weight: 36 kDa, 51-59 kDa
• GenBank Accession Number: BC017296
• Gene Symbol: SESN3
• Gene ID (NCBI): 143686
• RRID: AB_2185602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P58005
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Publications

What published studies show

Catalog #11431-2-AP has 22 citations published across 20 journals, including Theranostics, Cell Death & Disease, PNAS, Developmental Cell, eLife, and Biochimica et Biophysica Acta. Research fields span aging and senescence, osteoarthritis, autophagy, mTOR signaling, exercise physiology, cancer (hepatic and pulmonary), epilepsy, metabolic regulation, and cardiovascular disease. Tested applications include WB, IF, and IHC in human, mouse, and rat models.

Automatically generated from the 22 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human, mouse	WB,IF	Theranostics; Super-enhancer-driven core transcription factor FOXP1 delays endothelial cell senescence via phase separation-mediated SESN3 activation.; Authors - Lushuang Mao
mouse	IHC	Pharmacol Res; ESC-sEVs alleviate non-early-stage osteoarthritis progression by rejuvenating senescent chondrocytes via FOXO1A-autophagy axis but not inducing apoptosis; Authors - Kai Feng
human	WB	Cell Death Dis; Inhibition of p21 activates Akt kinase to trigger ROS-induced autophagy and impacts on tumor growth rate; Authors - Mayank Maheshwari
mouse	WB	Cell Death Dis; Pifithrin-μ sensitizes mTOR-activated liver cancer to sorafenib treatment; Authors - Jiarui Lv
mouse	IHC	Proc Natl Acad Sci U S A; FOXO1 and FOXO3 transcription factors have unique functions in meniscus development and homeostasis during aging and osteoarthritis.; Authors - Kwang Il Lee
human	WB	Dev Cell; FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor.; Authors - Chia-Chen Chen ; KD Validated