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  • KD/KO Validated

SESN3 Polyclonal antibody

SESN3 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11431-2-AP

Synonyms

SESN3, SEST3, sestrin 3



Tested Applications

Positive WB detected inHeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

11431-2-AP targets SESN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SESN3 fusion protein Ag1961
Full Name sestrin 3
Calculated molecular weight 492 aa, 57 kDa
Observed molecular weight 51-59 kDa
GenBank accession numberBC017296
Gene symbol SESN3
Gene ID (NCBI) 143686
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Protocols

Product Specific Protocols
WB protocol for SESN3 antibody 11431-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor.

Authors - Chia-Chen Chen
  • KD Validated
mouseIHC

Proc Natl Acad Sci U S A

FOXO1 and FOXO3 transcription factors have unique functions in meniscus development and homeostasis during aging and osteoarthritis.

Authors - Kwang Il Lee
humanWB

Elife

Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation.

Authors - Veronique Nogueira
  • KD Validated
humanWB,IHC

Osteoarthritis Cartilage

Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.

Authors - T Shen
humanWB

PLoS One

Regulatory Effects of Sestrin 3 (SESN3) in BCR-ABL Expressing Cells.

Authors - Vakana Eliza E
humanWB

BMC Cancer

Inhibition of mTORC1 through ATF4-induced REDD1 and Sestrin2 expression by Metformin.

Authors - Se-Kyeong Jang