SFRS2 Polyclonal antibody

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  WB analysis using 20371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 20371-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 20371-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20371-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20371-1-AP

  IP result of anti-SFRS2 (IP:20371-1-AP, 4ug; Detection:20371-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 20371-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20371-1-AP (SFRS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20371-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20371-1-AP (SFRS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20371-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS2 antibody (20371-1-AP) at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

SFRS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20371-1-AP

Publications(15)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

Serine/arginine-rich splicing factor 2, SC-35, SC35, SC 35, Protein PR264

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse testis tissue, A549 cells, rat brain tissue, HepG2 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 5 publications below
• RIP: See 1 publications below

Product Information

20371-1-AP targets SFRS2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: splicing factor, arginine/serine-rich 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-35 kDa
• GenBank Accession Number: NM_003016
• Gene Symbol: SFRS2
• Gene ID (NCBI): 6427
• RRID: AB_2616311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01130
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS2, also named as PR264 and Splicing component, 35 kDa, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. The molecular weight of modified SFRS2 is 35-40kDa. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.

Publications

Species	Application	Title
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	EMBO Mol Med; SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity; Authors - Indranil Malik
human	WB,IF	Int J Biol Macromol; 5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2; Authors - Hanliang Sun
human,mouse	WB	Am J Cancer Res; tRF-20-M0NK5Y93-induced MALAT1 promotes colon cancer metastasis through alternative splicing of SMC1A; Authors - Na Luan ; KD Validated
human	IF	J Biol Chem; Proteomic study identifies Aurora-A mediated regulation of alternative splicing through multiple splicing factors; Authors - Arun Prasath Damodaran
human	WB	Neuroscience; The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain.; Authors - Obayed Raihan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Saeed (Verified Customer) (05-27-2025)

The SRSF2 antibody did not work well for IF. As SRSF2 is a nuclear speckle member, after doing IF for it, we will see nuclear speckles in the cells, but using this antibody, there were no speckles, and all the cells were green. But I used another antibody from another company, and I had those speckles. I have uploaded the image.

• Applications: Immunofluorescence

FH

Xingyuan (Verified Customer) (08-15-2023)

Very nice quality at 1:500 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: hek293t

SFRS2 Antibody Western Blot validation (1:500 dilution) in hek293t (Cat no:20371-1-AP)

FH

Xingyuan (Verified Customer) (07-17-2023)

The quality of SRSF2's antibody wasn't good. I was not able to see bands at around 37kDa (tried 3 times).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

SFRS2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:20371-1-AP)