Tested Applications
Positive WB detected in | NIH/3T3 cells, mouse testis tissue, A549 cells, rat brain tissue, HepG2 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1500-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 11 publications below |
IF | See 5 publications below |
RIP | See 1 publications below |
Product Information
20371-1-AP targets SFRS2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide Predict reactive species |
Full Name | splicing factor, arginine/serine-rich 2 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 25-35 kDa |
GenBank Accession Number | NM_003016 |
Gene Symbol | SFRS2 |
Gene ID (NCBI) | 6427 |
RRID | AB_2616311 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q01130 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SFRS2, also named as PR264 and Splicing component, 35 kDa, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. The molecular weight of modified SFRS2 is 35-40kDa. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.
Protocols
Product Specific Protocols | |
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WB protocol for SFRS2 antibody 20371-1-AP | Download protocol |
IHC protocol for SFRS2 antibody 20371-1-AP | Download protocol |
IF protocol for SFRS2 antibody 20371-1-AP | Download protocol |
IP protocol for SFRS2 antibody 20371-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. | ||
EMBO Mol Med SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity | ||
Int J Biol Macromol 5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2 | ||
Am J Cancer Res tRF-20-M0NK5Y93-induced MALAT1 promotes colon cancer metastasis through alternative splicing of SMC1A
| ||
J Biol Chem Proteomic study identifies Aurora-A mediated regulation of alternative splicing through multiple splicing factors | ||
Neuroscience The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Saeed (Verified Customer) (05-27-2025) | The SRSF2 antibody did not work well for IF. As SRSF2 is a nuclear speckle member, after doing IF for it, we will see nuclear speckles in the cells, but using this antibody, there were no speckles, and all the cells were green. But I used another antibody from another company, and I had those speckles. I have uploaded the image.
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FH Xingyuan (Verified Customer) (08-15-2023) | Very nice quality at 1:500 dilution.
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FH Xingyuan (Verified Customer) (07-17-2023) | The quality of SRSF2's antibody wasn't good. I was not able to see bands at around 37kDa (tried 3 times).
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