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- KD/KO Validated
SHARPIN Polyclonal antibody
SHARPIN Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, co-IP, ELISA
Conjugate
Unconjugated
Cat no : 14626-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human lymphoma tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 60 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 7 publications below |
Product Information
14626-1-AP targets SHARPIN in WB, IP, IHC, IF, co-IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SHARPIN fusion protein Ag6201 |
| Full Name | SHANK-associated RH domain interactor |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC025244 |
| Gene symbol | SHARPIN |
| Gene ID (NCBI) | 81858 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SHARPIN antibody 14626-1-AP | Download protocol |
| IHC protocol for SHARPIN antibody 14626-1-AP | Download protocol |
| IF protocol for SHARPIN antibody 14626-1-AP | Download protocol |
| IP protocol for SHARPIN antibody 14626-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell MST1 Negatively Regulates TNFα-Induced NF-κB Signaling through Modulating LUBAC Activity. | ||
Nat Commun Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation. | ||
Arch Oral Biol A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis. | ||
Cell Rep Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death. | ||
Commun Biol Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses. | ||
Cell Rep OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Katharina (Verified Customer) (07-17-2019) | The antibody worked as expected, detecting a nice band at the right size. Recommended
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