|Positive WB detected in||Raji cells, A549 cells, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human lymphoma tissue, human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
14626-1-AP targets SHARPIN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SHARPIN fusion protein Ag6201|
|Full Name||SHANK-associated RH domain interactor|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC025244|
|Gene ID (NCBI)||81858|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.
MST1 Negatively Regulates TNFα-Induced NF-κB Signaling through Modulating LUBAC Activity.
Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation.
Arch Oral Biol
A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.
Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.
Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses.
Cell Chem Biol
A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Katharina (Verified Customer) (07-17-2019)
The antibody worked as expected, detecting a nice band at the right size. Recommended