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  • KD/KO Validated

SHARPIN Polyclonal antibody

SHARPIN Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14626-1-AP

Synonyms

hSIPL1, SHARPIN, SIPL1



Tested Applications

Positive WB detected inRaji cells, A549 cells, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

14626-1-AP targets SHARPIN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHARPIN fusion protein Ag6201
Full Name SHANK-associated RH domain interactor
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC025244
Gene symbol SHARPIN
Gene ID (NCBI) 81858
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHARPIN antibody 14626-1-APDownload protocol
IHC protocol for SHARPIN antibody 14626-1-APDownload protocol
IP protocol for SHARPIN antibody 14626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

MST1 Negatively Regulates TNFα-Induced NF-κB Signaling through Modulating LUBAC Activity.

Authors - In Young Lee
mouseWB

Nat Commun

Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation.

Authors - Yves Dondelinger
humanWB

Arch Oral Biol

A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.

Authors - Shichen Sun

Cell Rep

Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.

Authors - Todd Douglas
humanWB

Commun Biol

Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses.

Authors - Daisuke Oikawa
humanWB

Cell Chem Biol

A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN.

Authors - Aurelia Weber

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Katharina (Verified Customer) (07-17-2019)

The antibody worked as expected, detecting a nice band at the right size. Recommended

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549 cells /immunoprecipitates