A549 cells were subjected to SDS PAGE followed by western blot with 14626-1-AP(SHARPIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 14626-1-AP( SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP( SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP( SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Raji cells were subjected to SDS PAGE followed by western blot with 14626-1-AP( SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP( SHARPIN Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP( SHARPIN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP( SHARPIN Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP( SHARPIN Antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-SHARPIN (IP:14626-1-AP, 3ug; Detection:14626-1-AP 1:1500) with Raji cells lysate 2520ug.