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SHARPIN Antibody 39 Publications

Rabbit Polyclonal| Catalog number: 14626-1-AP

Featured Product KD/KO validated

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Con: 49 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

SHARPIN Antibody WB Raji cells 14626-1-AP

Raji cells were subjected to SDS PAGE followed by western blot with 14626-1-AP(SHARPIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

SHARPIN Antibody IP Raji cells 14626-1-AP

IP Result of anti-SHARPIN (IP:14626-1-AP, 3ug; Detection:14626-1-AP 1:1500) with Raji cells lysate 2520ug.

SHARPIN Antibody IHC human lymphoma tissue 14626-1-AP

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14626-1-AP( SHARPIN Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

SHARPIN Antibody IHC human lymphoma tissue 14626-1-AP

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14626-1-AP( SHARPIN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
Raji cells, A549 cells, Jurkat cells
Positive IP detected in:
Raji cells
Positive IHC detected in:
human lymphoma tissue, human small intestine tissue
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
IHC : 1:100-1:400

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SHARPIN fusion protein Ag6201
Full name:
SHANK-associated RH domain interactor
Calculated molecular weight:
40 kDa
Observed molecular weight:
40 kDa
GenBank accession number:
BC025244
Gene ID (NCBI):
81858
Gene symbol
SHARPIN
Synonyms
hSIPL1, SHARPIN, SIPL1

Background

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.


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