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SHARPIN Polyclonal antibody

SHARPIN Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14626-1-AP

Synonyms

hSIPL1, SHARPIN, SIPL1



Tested Applications

Positive WB detected inA549 cells, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14626-1-AP targets SHARPIN in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHARPIN fusion protein Ag6201
Full Name SHANK-associated RH domain interactor
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC025244
Gene symbol SHARPIN
Gene ID (NCBI) 81858
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHARPIN antibody 14626-1-APDownload protocol
IHC protocol for SHARPIN antibody 14626-1-APDownload protocol
IF protocol for SHARPIN antibody 14626-1-APDownload protocol
IP protocol for SHARPIN antibody 14626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

OTULIN limits cell death and inflammation by deubiquitinating LUBAC.

Authors - Klaus Heger
mouseWB,IP

Nature

LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.

Authors - Nieves Peltzer
humanWB,IP

Cell

OTULIN Antagonizes LUBAC Signaling by Specifically Hydrolyzing Met1-Linked Polyubiquitin.

Authors - Keusekotten Kirstin K
humanWB

Cell

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Authors - Rune Busk Damgaard
mouseWB

Cancer Cell

A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.

Authors - Yannick Boege
mouseWB

Nat Immunol

Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.

Authors - Yang Shuo S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Katharina (Verified Customer) (07-17-2019)

The antibody worked as expected, detecting a nice band at the right size. Recommended

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549 cells /immunoprecipitates