Tested Applications
| Positive WB detected in | A549 cells, mouse brain tissue, mouse skeletal muscle tissue, Jurkat cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human lymphoma tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14626-1-AP targets SHARPIN in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6201 Product name: Recombinant human SHARPIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC025244 Sequence: MAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGATVEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPAQLVLSFLHLHQCPRPPWL Predict reactive species |
| Full Name | SHANK-associated RH domain interactor |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC025244 |
| Gene Symbol | SHARPIN |
| Gene ID (NCBI) | 81858 |
| RRID | AB_2187734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0F6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SHARPIN antibody 14626-1-AP | Download protocol |
| IHC protocol for SHARPIN antibody 14626-1-AP | Download protocol |
| IP protocol for SHARPIN antibody 14626-1-AP | Download protocol |
| WB protocol for SHARPIN antibody 14626-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SHARPIN Polyclonal antibody (catalog no. 14626-1-AP) has 124 citations. They appear across high-impact journals including Nature, Cell, Immunity, Nature Immunology, Nature Communications, Molecular Cell, Cell Death & Differentiation, EMBO Journal, Cell Reports, PNAS, and Journal of Experimental Medicine. Research fields center on linear ubiquitination and the LUBAC complex, NF-κB signaling, RIPK1-mediated cell death, inflammation and autoinflammation, innate immunity, and cancer (hepatocellular, colorectal, gastric, and renal carcinomas), with applications mainly in Western blot and co-immunoprecipitation.
| Species | Application | Title |
|---|---|---|
Nature LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. | ||
Cancer Cell A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development. | ||
Cell The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. | ||
Cell OTULIN Antagonizes LUBAC Signaling by Specifically Hydrolyzing Met1-Linked Polyubiquitin. |
Reviews
What customers say
The single review for this product is positive, with a 4-star rating. The user performed a Western Blot at a 1:1000 dilution on A549 cell immunoprecipitates and reported that the antibody performed as expected, producing a clear band at the correct molecular weight. The reviewer recommended the product.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Katharina (Verified Customer) (07-17-2019) | The antibody worked as expected, detecting a nice band at the right size. Recommended
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