Validation Data Gallery
|Positive WB detected in||A549 cells, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue, Raji cells|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human lymphoma tissue, human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14626-1-AP targets SHARPIN in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SHARPIN fusion protein Ag6201|
|Full Name||SHANK-associated RH domain interactor|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC025244|
|Gene ID (NCBI)||81858|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for SHARPIN antibody 14626-1-AP||Download protocol|
|IHC protocol for SHARPIN antibody 14626-1-AP||Download protocol|
|IF protocol for SHARPIN antibody 14626-1-AP||Download protocol|
|IP protocol for SHARPIN antibody 14626-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Katharina (Verified Customer) (07-17-2019)
The antibody worked as expected, detecting a nice band at the right size. Recommended